Simultaneous Measurement of C14 and H3 during Gas-Liquid Chromatography
Gas-liquid chromatography (GLC), developed by James and Martin , is now recognized as the most powerful technique available for the separation of volatile substances of a homologous series. It is used not only for the analysis of the composition of natural products, but is also employed with increasing frequency for the identification of substances. It is a particularly valuable tool for biochemists studying biosynthetic processes, as its speed and sensitivity enable one to identify with considerable confidence new products on a minute scale. As biochemists often use isotopically labeled compounds in their investigations, the problem of simultaneous gas-liquid chromatographic analysis and monitoring of the chromatographic vapors for a radioactive isotope arose quite naturally. It is a common practice in many laboratories to use some kind of automatic device for the scanning of paper chromatograms for radioactive spots, but the monitoring of the effluent from gas-liquid chromatographic columns for a radioactive isotope has been achieved so far only in a few laboratories.
KeywordsScintillation Counter Counting Chamber Liquid Scintillator Injection Nozzle Counting Level
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