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Simultaneous Measurement of C14 and H3 during Gas-Liquid Chromatography

  • G. Popjak
  • A. E. Lowe
  • D. Moore

Abstract

Gas-liquid chromatography (GLC), developed by James and Martin [1], is now recognized as the most powerful technique available for the separation of volatile substances of a homologous series. It is used not only for the analysis of the composition of natural products, but is also employed with increasing frequency for the identification of substances. It is a particularly valuable tool for biochemists studying biosynthetic processes, as its speed and sensitivity enable one to identify with considerable confidence new products on a minute scale. As biochemists often use isotopically labeled compounds in their investigations, the problem of simultaneous gas-liquid chromatographic analysis and monitoring of the chromatographic vapors for a radioactive isotope arose quite naturally. It is a common practice in many laboratories to use some kind of automatic device for the scanning of paper chromatograms for radioactive spots, but the monitoring of the effluent from gas-liquid chromatographic columns for a radioactive isotope has been achieved so far only in a few laboratories.

Keywords

Scintillation Counter Counting Chamber Liquid Scintillator Injection Nozzle Counting Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • G. Popjak
    • 1
  • A. E. Lowe
    • 1
  • D. Moore
    • 1
  1. 1.Medical Research Council Experimental Radiopathology Research UnitHammersmith HospitalLondonEngland

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