Scintillation Counting of C14-Labeled Paper Chromatograms
If one is faced with the problem of determining the distribution of radioactivity in a paper chromatogram, there are several assay techniques available. The chromatogram may be scanned in a strip counter and the radioactivity estimated by measuring areas under each peak or by use of an electronic integrator . The paper strip can also be cut into appropriate sections and the radioactivity of each section determined under a Geiger counter. Depending on the thickness of the paper, the counting efficiency of these techniques is in the range of 3–10%. More serious than the inefficiency is the variation in efficiency, which makes quantitation difficult.
KeywordsSteam Valine Haas Isoleucine H2NOH
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