Scintillation Counting of C14-Labeled Paper Chromatograms

  • Robert Berner Loftfield

Abstract

If one is faced with the problem of determining the distribution of radioactivity in a paper chromatogram, there are several assay techniques available. The chromatogram may be scanned in a strip counter and the radioactivity estimated by measuring areas under each peak or by use of an electronic integrator [1]. The paper strip can also be cut into appropriate sections and the radioactivity of each section determined under a Geiger counter. Depending on the thickness of the paper, the counting efficiency of these techniques is in the range of 3–10%. More serious than the inefficiency is the variation in efficiency, which makes quantitation difficult.

Keywords

Steam Valine Haas Isoleucine H2NOH 

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References

  1. (1).
    Ludwig, H., Potter, V.R., Heidelberger, C., and de Verdier, C.H., Biochim. et Biophysica Acta 37, 5–25 (1960).CrossRefGoogle Scholar
  2. (2).
    Wang, C.H. and Jones, D.E., Research Communications 1, 203 (1959).Google Scholar
  3. (3).
    Loftfield, R. B. and Eigner, E. A., J. Am. Chem. Soc. 81, 4753 (1959).Google Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Robert Berner Loftfield
    • 1
  1. 1.J. Collins Warren Laboratories of the Huntington Memorial Hospital of Harvard UniversityMassachusetts General HospitalBostonUSA

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