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Preparation of Tritium-Labeled Paromomycin (Humatin) by Fermentation in a Medium Containing Tritiated Water

  • Robert E. Ober
  • Salvatore A. Fusari
  • George L. Coffey
  • G. Wayne Gwynn
  • Anthony J. Glazko

Abstract

The antibiotic paromomycin is a basic, water-soluble compound with a general structure similar to that of neomycin and kanamycin [1]. Failure to obtain radiochemically pure paromomycin following exposure to tritium gas by the Wilzbach technique [2] prompted us to try biosynthesis. While biosynthetic incorporation of hydrogen isotope from water into organic compounds has been reported frequently in the literature [3–5], this technique has not been used widely for preparation of tritium-labeled compounds. While the work reported here was in progress, Dutton et al. [6] reported the isolation of tritium-labeled fatty acids from soybeans grown in a nutrient solution containing tritiated water.

Keywords

Hydrogen Isotope Sulfate Salt Tritiated Water Label Material Pool Urine Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Dutton, H. J., Jones, E. P., Scholfield, C.R., Chorney, W., and Scully, M J. J. Lipid Research 2, 63 (1961).Google Scholar
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    Coffey, G. L., Anderson, L. E., Fisher, M. W., Galbraith, M.M., Hillegas, A.B., Koh-berger, D. L., Thompson, P. E., Weston, K. S., and Ehrlich, J. Antibiotics and Chemotherapy 9, 730 (1959).Google Scholar
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    Kelly, R. G., Peets, E. A., Gordon, S., and Buyske, D.A. Anal. Biochem. 2, 267 (1961).CrossRefGoogle Scholar

Copyright information

© New England Nuclear Corporation 1963

Authors and Affiliations

  • Robert E. Ober
    • 1
  • Salvatore A. Fusari
    • 1
  • George L. Coffey
    • 1
  • G. Wayne Gwynn
    • 1
  • Anthony J. Glazko
    • 1
  1. 1.Research DivisionParke, Davis & CompanyAnn Arbor and DetroitUSA

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