Preparation of Tritium-Labeled Paromomycin (Humatin) by Fermentation in a Medium Containing Tritiated Water
The antibiotic paromomycin is a basic, water-soluble compound with a general structure similar to that of neomycin and kanamycin . Failure to obtain radiochemically pure paromomycin following exposure to tritium gas by the Wilzbach technique  prompted us to try biosynthesis. While biosynthetic incorporation of hydrogen isotope from water into organic compounds has been reported frequently in the literature [3–5], this technique has not been used widely for preparation of tritium-labeled compounds. While the work reported here was in progress, Dutton et al.  reported the isolation of tritium-labeled fatty acids from soybeans grown in a nutrient solution containing tritiated water.
KeywordsHydrogen Isotope Sulfate Salt Tritiated Water Label Material Pool Urine Sample
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