Photoregulation of LHC-II Accumulation in Thylakoids during Chloroplast Development

  • J. H. Argyroudi-Akoyunoglou
Part of the NATO ASI Series book series (NSSA, volume 168)


The biogenesis of the photosynthetic units depends on close cooperation of the chloroplast and cytoplasm protein synthesizing machinery:the reaction center (RC) core proteins are coded by the plastid DNA, synthesized on thylakoid-bound ribosomes, and cotranslationaly inserted into the developing membrane; on the other hand, the light-harvesting antennae apoprotein LHC-II, is coded by nuclear DNA, synthesized on cytoplasmic ribosomes, as higher molecular weight hydrophilic precursor, it is post-translationally imported through the plastid envelope, and after its processing to its mature size, by cleavage of its transient sequence, it is incorporated into the thylakoid. No precursor or mature form have been detected in the envelope or stroma of the chloroplast, suggesting a rapid transport through the various compartments. A small amount of precursor LHC-II apoprotein has been found in the thylakoid recently, suggesting that processing may also take place in the thylakoid1–3. Stabilization of the mature hydrophobic protein in the thylakoid seems to require concomitant Chlorophyll synthesis, which allows its stabilization via Chl-protein complex formation.


Continuous Light Unit Formation Unit Content Reaction Center Protein PSII Unit 
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  1. 1.
    R. J. Ellis, Chlorophyll-proteins: Synthesis, transport and assembly, Ann Rev Plant Physiol., 32:111 (1981).CrossRefGoogle Scholar
  2. 2.
    N. H. Chua and G.W. Schmidt, Transport of proteins into mitochondria and chloroplasts, J. Cell Biol. 81:461 (1979).PubMedCrossRefGoogle Scholar
  3. 3.
    P. R. Chitnis et al., Assembly of the precursor and processed Lightharvesting Chla/b protein of Lemna into the Light-harvesting Complex II of barley etiochloroplasts, J. Cell Biol. 102:982 (1986).PubMedCrossRefGoogle Scholar
  4. 4.
    K. Apel, Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare) , Eur. J Biochem. 97:183 (1979).PubMedCrossRefGoogle Scholar
  5. 5.
    P. Tavladoraki, G. Akoyunoglou, A. Bitsch, G. Meyer and K. Kloppstech, Age and Phytochrome-induced changes at the level of the translatable mRNA coding for the LHC-II apoprotein of Phaseolus vulgaris leaves, in:“Regulation of Chloroplast Differentiation”, G. Akoyunoglou and H. Senger eds., Liss, N.Y. pp. 559–564 (1986).Google Scholar
  6. 6.
    P. Tavladoraki, K. Kloppstech and J.H. Argyroudi-Akoyunoglou, Circadian rhythm in the expression of the mRNA coding for the apoprotein of the light-harvesting complex of Photosystem II: Phytochrome control and persistent Far Red reversibility, Plant Physiol in press (1988).Google Scholar
  7. 7.
    J. H. Argyroudi-Akoyunoglou and G. Akoyunoglou, Photoinduced changes in the Chlorophyll a to Chlorophyll b ratio in young bean plants, Plant Physiol., 46:247 (1970).PubMedCrossRefGoogle Scholar
  8. 8.
    J. H. Argyroudi-Akoyunoglou and G. Akoyunoglou, The Chlorophyll-protein complexes of thylakoids in greening plastids of Phaseolus vulgaris, FEBS Lett., 104:78 (1979)CrossRefGoogle Scholar
  9. 9.
    G. Akoyunoglou, Development of the Photosystem II unit in plastids of bean leaves greened in periodic light, Arch. Biochem. Biophys., 183:571 (1977).PubMedCrossRefGoogle Scholar
  10. 10.
    G. Akoyunoglou, Assembly of functional components in chloroplast photosynthetic membranes, in: Photosynthesis, G. Akoyunoglou ed., Vol. V, Balaban, Pa., pp. 353–366 (1981).Google Scholar
  11. 11.
    J. Bennett, Biosynthesis of the light-harvesting Chla/b protein. Polypeptide turnover in darkness. Eur. J. Biochem. 118:61 (1981).PubMedCrossRefGoogle Scholar
  12. 12.
    J. H. Argyroudi-Akoyunoglou, A. Akoyunoglou, K. Kalosakas and G. Akoyunoglou, Reorganization of the PSII unit in developing thylakoids of higher plants after transfer to darkness: Changes in Chl b, Light-harvesting Chl-protein content, and grana stacking. Plant Physiol., 70:1242 (1982).PubMedCrossRefGoogle Scholar
  13. 13.
    A. Akoyunoglou, and G. Akoyunoglou, Reorganization of thylakoid components during chloroplast development in higher plants after transfer to darkness. Changes in PSI unit components and in cytochromes, Plant Physiol. 79:425 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    G. Akoyunoglou, Biosynthesis, Assembly and properties of the pigment-protein Complexes, in: Proc. 16th FEBS Congress, VNU Sci:Press, Utrecht, pp 35–46 (1985).Google Scholar
  15. 15.
    G. Akoyunoglou and J.H. Argyroudi-Akoyunoglou, Post-translational regulation of chloroplast differentiation, in:Regulation of Chloroplast Differentiation, G. Akoyunoglou and H. Senger eds., Liss, N.Y., pp. 571–582 (1986).Google Scholar
  16. 16.
    G. Tzinas, G. Akoyunoglou and J.H. Argyroudi-Akoyunoglou, The effect of the dark interval in intermittent light on thylakoid development: photosynthetic unit formation and light-harvesting protein accumulation, Photosynthesis Res., 14:241 (1987).CrossRefGoogle Scholar
  17. 17.
    G. Tzinas and J.H. Argyroudi-Akoyunoglou, Chloramphenicol-induced stabilization of light-harvesting complexes in thylakoids during development, FEBS Lett., 229:135 (1988).CrossRefGoogle Scholar
  18. 18.
    G. Akoyunoglou and J.H. Argyroudi-Akoyunoglou, Control of thylakoid growth in Phaseolus vulgaris, Plant Physiol. 61:834 (1978).PubMedCrossRefGoogle Scholar
  19. 19.
    S. Tsakiris and G. Akoyunoglou, Formation and growth of Photosystem I and II units in old bean leaves, Plant Sci. Lett., 35:97 (1984).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • J. H. Argyroudi-Akoyunoglou
    • 1
  1. 1.NRCPS “Demokritos”AthensGreece

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