Subfractional Analysis of Thylakoid Membrane Organization and Adaptation
In Dutch the word for chemistry is scheikunde. This is one illustration of the central role for the separation concept in experimental chemistry. The need for efficient fractionation methods does also apply to biochemistry and photo-synthetic research. Not until pure and intact chloroplasts were isolated in the fifties could the subcellular localization of CO2 fixation be established1. Thylakoid preparations completely free of contaminating mitochondria were a prerequisite for proving the existence of photophosphorylation1. Today we see the importance of subfractionation for the isolation of thylakoid protein complexes with a defined function and composition2. For the recent dramatic progress in our understanding of photosystem II, subfractionation has been a real cornerstone. In this paper we describe the sub-fractional approach for studies on the organization and dynamics of the thylakoid membrane.
KeywordsThylakoid Membrane Antenna Size Spinach Thylakoid Stroma Thylakoid Thylakoid Vesicle
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