Chloroplast Development in T-DNA Gene 4 Transformed Calli
The first results are presented of a study on chloroplast development in transformed cell lines obtained by incorporation of chimeric T-DNA gene 4 constructions in the genome of Nicotiana tabacum cv. petit Havanna SRI (gene 4 is coding for isopentenyltransferase). These chimeric genes were constructed in the pGV831 vector and introduced in the Ti-plasmid vector pGV 2260 of Agrobacterium tumefaciens1. Since pGV831 contained a selectable marker (Pnos-nptll, resistance against kanamycine) the transformed cells could be selected on a medium containing kanamycin and both auxins (IAA) and cyto-kinins (BAP). This procedure allowed to compare the activity of different chimeric genes in an identical background. The preliminary experiments indicate that the development of functional chloroplasts is cytokinin (ZR) dependent.
KeywordsControl Line Chimeric Gene Chloroplast Development Transform Cell Line Zeatine Riboside
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