Characterization of cDNA Clones Encoding the Pea Chloroplast Rieske Fe-S Protein

  • A. Hugh Salter
  • Barbara J. Newman
  • John C. Gray
Part of the NATO ASI Series book series (NSSA, volume 168)


The cytochrome b-f complex of the chloroplast thylakoid membrane operates as a plastoquinol/plastocyanin oxido-reductase and is thus analogous to the mitochondrial cytochrome bc 1 complex with which it bears a large amount of structural and functional similarity1 It consists of four components, which are cytochrome f, cytochrome b-563, the Rieske Fe-S protein, and a 17kDa protein (subunit IV). In addition a number of small (~5kDa) have been suggested to be part of the complex2, though this requires confirmation. The Rieske Fe-S protein may be removed from the isolated complex by hydroxyapatite chromatography in the presence of Triton X-1003 and may be identified by a characteristic EPR signal4 as well as by a size of 20kDa on SDS-PAGE.


Spinach Chloroplast 26kDa Precursor Chloroplast Thylakoid Membrane Hydroxyapatite Chromatography Nuclear Encode Chloroplast Proteins13 


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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • A. Hugh Salter
    • 1
  • Barbara J. Newman
    • 1
  • John C. Gray
    • 1
  1. 1.Botany SchoolUniversity of CambridgeUK

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