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Coarse Control of Sucrose-Phosphate Synthase

  • Gabriele Siegl
  • Mark Stitt
Part of the NATO ASI Series book series (NSSA, volume 168)

Abstract

In green plant tissue the photosynthate is transported to the sink regions of the plant as sucrose. In order to investigate plant physiology it is of great interest to look at the regulation of sucrose synthesis. In the reaction sequence leading to sucrose one of the steps that are thought to be regulated is the reaction catalized by sucrose-phosphate synthase in which fructose-6-phosphate and UDP-glucose are converted to sucrose-phosphate and UDP. When people measure sucrose-phosphate synthase activity in spinach leaves at different times of a day they find a diurnal rhythm when the reaction is assayed using conditions that correspond to in vivo concentrations. A diurnal rhythm measured under Vmax conditions, however, results in constant activities over 24 hours. Thus the properties of sucrose-phosphate synthase change during the day: In the morning the enzyme shows high activity that is hardly influenced by physiological phosphate concentrations and in the evening it has low activity that is inhibited by physiological phosphate concentrations, only leaving a residual activity of about 10%. We now wish to characterize these two forms of sucrose-phosphate synthase, whose relative amount change in leaves and produce the diurnal changes. We aim to purify the enzyme so far that kinetic studies can be done.

Keywords

Diurnal Rhythm Diurnal Change Constant Activity Fast Protein Liquid Chromatography Sucrose Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literature

  1. Stitt, M., Wilke, I., and Heldt, H.W., (1988), Coarse Control of Sucrose-Phosphate Synthase in Leaves: Alterations of the kinetic properties in response to the rate of photosynthesis and the accumlation of sucrose. Planta 174:217–230CrossRefGoogle Scholar
  2. Stitt, M., Huber, S., and Kerr, P., (1987), Control of photosynthetic sucrose formation In: The Biochemistry of Plants, Vol. 10, pp. 327–409, Hatch, M.D. and Boardman, N.K., eds.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Gabriele Siegl
    • 1
  • Mark Stitt
    • 1
  1. 1.PflanzenphysiologieUniversität BayreuthBayreuthGermany

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