Control of Sucrose Synthesis: Estimation of Free Energy Changes, Investigation of the Contribution of Equilibrium and Non-Equilibrium Reactions, and Estimation of Elasticities and Flux Control Coefficients

  • Mark Stitt
Part of the NATO ASI Series book series (NSSA, volume 168)


In this chapter I will review how ideas about the control of metabolism have developed in other fields, and will then apply these ideas to the regulation of photosynthetic sucrose synthesis. My main aim is to explore how control is organised and distributed between various enzymes, and how we can make quantitative statements about their response to effectors and their contribution to control in vivo. I will argue that a proper understanding of the nature of control is an essential prerequisite for the evalution of changes in flux and metabolite levels. In particular, it is essential to escape from an oversimplified use of the notion of ‘limitation’ in photosynthetic research.


Free Energy Change Metabolite Level Fractional Change Elasticity Coefficient Sucrose Synthesis 
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  1. Barber, T., G. 1985. The equlibrium of the reaction catalysed by sucrose P synthase, Plant Physiol 79, 1127–1128.PubMedCrossRefGoogle Scholar
  2. Bassham J.A. and Krause, G.H. (1969). Free energy changes and metabolic regulation in steady state photosynthetic carbon metabolism. Biochim. Biophys. Acta 189, 207–221.PubMedCrossRefGoogle Scholar
  3. Crabtree, B. and Newsholme, E.A. 1987. A systematic approach to describing and analysing metabolic control systems. Trends in Biochem. Sciences 12, 4–12.CrossRefGoogle Scholar
  4. Doehlert D.C. and Huber, S.C. 1988. Regulation of spinach leaf sucrose phosphate synthase by gluocose-6-phosphate, inorganic phosphate and pH.Google Scholar
  5. Doehlert, D.C. and Huber S.C. 1984. Phosphate inhibition of spinach leaf sucrose phosphate synthase as affected by glu-cose-6-phosphate and phosphoglucoisomerase. Plant Physiol. 76, 250–253.PubMedCrossRefGoogle Scholar
  6. Dyson, J.E.D. and Noltman, E.A. 1963. The effect of pH and temperature on the kinetic parameters of phosphoglucose isomer-ase. J. Biol. Chem. 243, 1401–1414.Google Scholar
  7. Foyer, C.H., Furbank, R.T. and Walker, D.A., 1987. Interactions between ribulose-1,5-bisphosphate carboxylase and stromal metabolites I. Modulation of enzyme activity by Benson-Calvin cycle intermediates Biochim. Biophys. Acta 894, 157–164.CrossRefGoogle Scholar
  8. Gardemann, A., Stitt, M. and Heldt, H.W., 1983. Control of carbon dioxide fixation. Regulation of spinach ribulose-5-phosphate kinase by stromal metabolite levels. Biochim Biophys. Acta 722, 51–60.CrossRefGoogle Scholar
  9. Gerhardt, R., Stitt, M. and Heldt, H.W. 1987. Subcellular metabolite levels in spinach leaves. Plant Physiol. 83, 399–407.PubMedCrossRefGoogle Scholar
  10. Groen, A.K., Van der Meer, R., Westerhoff, H.V., Wanders, R.T.A., Akerboom, T.P.M., Tager, J.M. 1982a. Control of Metabolic Fluxes. In Metabolic Compartmentation, (ed. H. Sies) p 937. London Academic Press.Google Scholar
  11. Groen, A.K., Wanders, R.T.A., Westerhoff, H. V., van der Meer, R. and Tager, T.M., 1982b. Quantification of the contribution of various steps to the control of mitochondrial respiration. J. Biol. Chem. 257, 2754–2757.PubMedGoogle Scholar
  12. Heinrich, R. and Rapaport, T.A., 1974. A linear steady state treatment of enzymatic chanis. Eur. J. Biochem. 42, 89–120.PubMedCrossRefGoogle Scholar
  13. Herzog, B., Stitt, M., and Heldt, H.W., 1984. Control of photo-synthetic sucrose synthesis by fructose-2,6-bisphosphate III Properties of the cytosolic Fructose-1,6-bisphosphatase. Plant Physiol. 75, 561–565.PubMedCrossRefGoogle Scholar
  14. Isherwood, F.A. and Selvandran, R.C. 1970. A note of the occurence of nucleotides in strawberry leaves. Phytochemistry, 2265–2267.Google Scholar
  15. Jones, T.W.A., Pichersky, E. and Gottlieb, L.D., 1986. Enzyme activity in EMS-induced null mutations of duplicated genes encoding phosphoglucose isomerase in Clarkia. Genetics 113, 101–114.PubMedGoogle Scholar
  16. Kacser, H. and Burns, J.A. 1973. The control of flux. Symp. Soc. Exp. Biol. 27, 65–107.PubMedGoogle Scholar
  17. Kacser, H. and Burns, J.A. 1979. Molecular Democracy : who shares the controls? Biochem. Soc. Trans 7, 1149–1161.PubMedGoogle Scholar
  18. Kacser, H. and Porteus, J.W., 1987. Control of metabolism : what do we have to measure? Trends in Biochem. Sciences 12, 5–14.Google Scholar
  19. Kerr, P.S. and Huber, S.C., 1987. Coordinate control of sucrose formation in soybean leaves by sucrose phosphate synthase and fructose-2,6-bisphosphate. Planta 170, 197–204.CrossRefGoogle Scholar
  20. Lehringer, A.L., 1970. Biochemistry, Worth Publ., New York.Google Scholar
  21. Newsholme, E.A. and Start, C., 1973. Regulation in Metabolism. Wiley and Sons.Google Scholar
  22. Rolleston, F.S., 1972. A theoretical background to the use of measured intermediates in the study of the control of intermediary metabolism. Curr. Top Cell. Reg. 5, 47–75.Google Scholar
  23. Scheibe, R., 1987 NADP malate dehydrogenase in C-3 plants: regu-altion and role of a light-activated enzyme. Physiol. Plan-tarum 71, 393–400.CrossRefGoogle Scholar
  24. Sicher, R.C., Kremer, D.F. and Harris, W.G., 1986. Control of photosynthetic sucrose synthesis in barley primary leaves. Plant Physiol. 82, 15–18.PubMedCrossRefGoogle Scholar
  25. Stitt, M., Wirtz, W. and Heldt, H.W., 1980. Metabolite levels in the chloroplast and extrachloroplast compartments of spinach protoplasts Biochim. Biophys. Acta 593, 85–102.PubMedCrossRefGoogle Scholar
  26. Stitt, M., Wirtz, W. and Heldt, H.W., 1983. Regulation of sucrose synthesis by cytoplasmic fructose bisphosphatase and sucrose phosphate synthase during photosynthesis in response to varying light and carbon dioxide. Plant Physiol. 72, 767–774.PubMedCrossRefGoogle Scholar
  27. Stitt, M., Cseke, C. and Buchanan, B.B., 1984. Regulation of fructose-2,6-bisphosphate concentration in spinach leaves. Eur. T. Biochem. 143, 89–93.CrossRefGoogle Scholar
  28. Stitt, M., Herzog, H. and Heldt, H.W., 1985a. Control of photo-synthetic sucrose synthesis by fructose-2,6-bisphosphate V. Modulation of the cytosolic fructose-1,6-bisphosphatase in vitro Plant Physiol. 79, 590–598.CrossRefGoogle Scholar
  29. Stitt, M. and Heldt, H.W., 1985. Control of photosynthetic sucrose synthesis by fructose-2,6-bisphosphate VI. Regulation of the cytosolic fructose-1,6-bisphosphatase in vivo. Plant Physiol. 79, 599–608.PubMedCrossRefGoogle Scholar
  30. Stitt, M., Wirtz, W., Gerhardt, R., Heldt, H.W., Spencer, C., Walker, D.A., Foyer, C.H., 1985b. A comparative study of metabolite levels in plant leaf material in the dark. Planta 166, 354–364.CrossRefGoogle Scholar
  31. Stitt, M., Gerhardt, R., Wilke, I. and Heldt, H.W., 1987. The contribution of fructose-2,6-bisphosphate to the regulation of sucrose synthesis during photosynthesis. Physiol. Plantarem 69, 377–386.CrossRefGoogle Scholar
  32. Stitt, M., Wilke, I., Feil, R. and Heldt, H.W. (1988). Coarse control of sucrose phosphate synthase in leaves. Planta 174, 217–230.CrossRefGoogle Scholar
  33. Stitt, M. and Große, H., 1988. Interaction between sucrose synthesis and photosynthesis IV. Temperature dependent adjustment of the relation between sucrose synthesis and CO2 fixation. J. Plant Physiol, (in press).Google Scholar
  34. Stitt, M., 1989. Control analysis of photosynthesis sucrose synthesis: assignment of elasticity coefficients and flux control coefficients to the cytosolic fructose-1,6-bisphos-phatase and sucrose phosphate synthase. Phil, trans. Roy. Soc. Lond. (In Press).Google Scholar
  35. Woodrow, I.E., 1986. Control of the rate of photosynthetic carbon dioxide fixation. Biochim. biophys. Acta 851, 181–192.CrossRefGoogle Scholar
  36. Weiner, H., Stitt, M. and Heldt, H.W., 1987. Subcellular compart-mentation of pyrophosphate and alkaline pyrophosphatase in leaves. Biochim. Biophys. Acta 893, 13–21.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Mark Stitt
    • 1
  1. 1.Lehrstuhl für PflanzenphysiologieUniversität BayreuthBayreuthGermany

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