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Light Driven Amino Acid Uptake in Membrane Vesicles of Streptococcus Cremoris Fused with Liposomes Containing Bacterial Reaction Centers

  • Wim Crielaard
  • Arnold J. M. Driessen
  • Douwe Molenaar
  • Klaas J. Hellingwerf
  • Wil N. Konings
Part of the NATO ASI Series book series (NSSA, volume 168)

Abstract

The incorporation of primary proton pumps in biological membranes has opened attractive possibilities for studies of proton motive force (Δp)-dependent processes in isolated membrane vesicles from bacterial1, and eukaryotic2 origin. Fused membranes obtained from liposomes containing a Ap-generating system and membrane vesicles of fermentative bacteria lacking an accessible proton pump have been shown to be excellent model systems for studies on the role of the Δp in solute transport1,3,5,6. Cytochrome c oxidase1 and bacteriorhodopsin3 have been used extensively as Δp-generating systems in these fused membranes.

Keywords

Reaction Center Membrane Vesicle Proton Motive Force Cyclic Electron Flow Cyclic Electron Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. 1.
    A.J.M. Driessen, W. de Vrij, and W.N. Konings, Incorporation of beef-heart cytochrome c oxidase as a proton-motive-force-generating mechanism in bacterial membrane vesicles, Proc. Natl. Acad. Sci. USA. 82:7555 (1985).PubMedCrossRefGoogle Scholar
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    A.J.M. Driessen, K.J. Hellingwerf, and W.N. Konings, Mechanism of energy coupling to entry and exit of neutral and branched chain amino acids in membrane vesicles of Streptococcus cremoris, J. Biol. Chem. 262:12438 (1987).PubMedGoogle Scholar
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Wim Crielaard
    • 1
  • Arnold J. M. Driessen
    • 1
  • Douwe Molenaar
    • 1
  • Klaas J. Hellingwerf
    • 1
  • Wil N. Konings
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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