Abstract
The proton channel of the chloroplast ATP synthase, CF0, was investigated by a flash spectrophotometric method, applied to a suspension of CFi depleted vesicles derived from thylakoid membranes (Lili, Engelbrecht, Schönknecht & Junge, 1986). Excitation of the vesicle suspension with short flashes of light generated an electric potential across the thylakoid membranes and a protonic charge pulse inside the vesicles. The relaxation of the voltage, due to ion fluxes across the membrane, was measured via electrochromic absorption changes of intrinsic pigments. It was compared with the proton fluxes which were determined by pH-indicating dyes in the lumen and in the medium.
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© 1989 Plenum Press, New York
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Althoff, G., Lill, H., Junge, W. (1989). The Single Channel Conductance of CF0 . In: Barber, J., Malkin, R. (eds) Techniques and New Developments in Photosynthesis Research. NATO ASI Series, vol 168. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8571-4_35
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DOI: https://doi.org/10.1007/978-1-4684-8571-4_35
Publisher Name: Springer, Boston, MA
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