Advertisement

The Single Channel Conductance of CF0

  • Gerd Althoff
  • Holger Lill
  • Wolfgang Junge
Part of the NATO ASI Series book series (NSSA, volume 168)

Abstract

The proton channel of the chloroplast ATP synthase, CF0, was investigated by a flash spectrophotometric method, applied to a suspension of CFi depleted vesicles derived from thylakoid membranes (Lili, Engelbrecht, Schönknecht & Junge, 1986). Excitation of the vesicle suspension with short flashes of light generated an electric potential across the thylakoid membranes and a protonic charge pulse inside the vesicles. The relaxation of the voltage, due to ion fluxes across the membrane, was measured via electrochromic absorption changes of intrinsic pigments. It was compared with the proton fluxes which were determined by pH-indicating dyes in the lumen and in the medium.

Keywords

Thylakoid Membrane Turnover Number Proton Flux Single Channel Conductance Isotopic Substitution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. A. Brünger, Z. Schulten & K. Schulten, 1983, Z.Phys.Chem. 136: 1–63CrossRefGoogle Scholar
  2. U. Junesch & P. Gräber, 1985, Biochim.Biophys.Acta 809: 429–434CrossRefGoogle Scholar
  3. W. Junge, B. Rumberg & H. Schröder, 1970, Eur.J.Biochem. 14: 575–581PubMedCrossRefGoogle Scholar
  4. W. Junge, 1987, Proc.Natl.Acad.Sci. USA 48: 7084–7088CrossRefGoogle Scholar
  5. H. Lill, S. Engelbrecht, G. Schönknecht & W. Junge, 1986, Eur.J.Biochem. 160: 627–634PubMedCrossRefGoogle Scholar
  6. H. Lill, G. Althoff, and W. Junge, 1987, J.Membrane Biol. 98: 69–78CrossRefGoogle Scholar
  7. J.F. Nagle & H.J. Morrowitz, 1978, Proc.Natl.Acad.Sci. USA 75: 298–302PubMedCrossRefGoogle Scholar
  8. E. Schneider & K. Altendorf, 1987, Microbiological Reviews 51: 477–497PubMedGoogle Scholar
  9. G. Schönknecht, W. Junge, H. Lill & S. Engelbrecht, 1986, FEBS Lett. 203: 289–294CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Gerd Althoff
    • 1
  • Holger Lill
    • 1
  • Wolfgang Junge
    • 1
  1. 1.BiophysikUniversität OsnabrückW. Germany

Personalised recommendations