The Single Channel Conductance of CF0
Part of the
NATO ASI Series
book series (NSSA, volume 168)
The proton channel of the chloroplast ATP synthase, CF0, was investigated by a flash spectrophotometric method, applied to a suspension of CFi depleted vesicles derived from thylakoid membranes (Lili, Engelbrecht, Schönknecht & Junge, 1986). Excitation of the vesicle suspension with short flashes of light generated an electric potential across the thylakoid membranes and a protonic charge pulse inside the vesicles. The relaxation of the voltage, due to ion fluxes across the membrane, was measured via electrochromic absorption changes of intrinsic pigments. It was compared with the proton fluxes which were determined by pH-indicating dyes in the lumen and in the medium.
KeywordsThylakoid Membrane Turnover Number Proton Flux Single Channel Conductance Isotopic Substitution
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© Plenum Press, New York 1989