Advertisement

Isolation of the Chlorophyll a/b Protein Complex CP29

  • Wolfgang P. Schröder
  • Michael Spangfort
  • Tomas Henrysson
  • Hans-Erik Akerlund
Part of the NATO ASI Series book series (NSSA, volume 168)

Abstract

The light harvesting apparatus of Photosystem II (PSII) has at least three chlorophyll a/b binding protein complexes. Apart from the most abundant complex, LHCII, there are the minor CP29 and CP24. The polypeptide composition and structure of CP24 have just recently been extensively characterized (Spangfort et al. these proceedings) but the precise structure and function of CP29 is still unknown. CP29 is normally isolated by partly denaturating sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) and consists of one polypeptide with an apparent molecular weight of 29 kDa which is unrelated to other chlorophyll-binding proteins (1). In this work we report a new procedure for the isolation of CP29 by mild detergent treatement of washed thylakoid membranes followed by a single HPLC step.

Keywords

Apparent Molecular Weight Photosynthesis Research PSII Membrane CP29 Protein PSII Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Green, B.R. (1988) Photosynthesis Research 15, 3–32CrossRefGoogle Scholar
  2. 2.
    Ford, R.C. and Evans, M.C.W. (1983) FEBS Lett 160, 159–164CrossRefGoogle Scholar
  3. 3.
    Ljungberg, U., Åkerlund, H.-E. and Andersson, B. (1986) Eur. J. Biochem. 158, 477–482PubMedCrossRefGoogle Scholar
  4. 4.
    Laemmli, U.K. (1970) Nature 227, 680–685PubMedCrossRefGoogle Scholar
  5. 5.
    Matsudaira, P. (1987) J. Biol. Chem. 262, 10035–10038PubMedGoogle Scholar
  6. 6.
    Dunahay, T.G., Schuster, G. and Staehelin, L.A. (1987) FEBS Lett. 215, 25–30PubMedCrossRefGoogle Scholar
  7. 7.
    Bassi, R. and Simpson, D. (1987) Progress in Photosynthesis Research (Biggens, J. ed.), Vol II, 81–88CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Wolfgang P. Schröder
    • 1
  • Michael Spangfort
    • 1
  • Tomas Henrysson
    • 1
  • Hans-Erik Akerlund
    • 1
  1. 1.Department of BiochemistryUniversity of LundLundSweden

Personalised recommendations