Isolation of the Chlorophyll a/b Protein Complex CP29
The light harvesting apparatus of Photosystem II (PSII) has at least three chlorophyll a/b binding protein complexes. Apart from the most abundant complex, LHCII, there are the minor CP29 and CP24. The polypeptide composition and structure of CP24 have just recently been extensively characterized (Spangfort et al. these proceedings) but the precise structure and function of CP29 is still unknown. CP29 is normally isolated by partly denaturating sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) and consists of one polypeptide with an apparent molecular weight of 29 kDa which is unrelated to other chlorophyll-binding proteins (1). In this work we report a new procedure for the isolation of CP29 by mild detergent treatement of washed thylakoid membranes followed by a single HPLC step.
KeywordsApparent Molecular Weight Photosynthesis Research PSII Membrane CP29 Protein PSII Particle
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