Long-Term Effects of Ribose on Adenine Nucleotide Metabolism in Isoproterenol-Stimulated Hearts

  • H.-G. Zimmer
  • H. Ibel
  • G. Steinkopff
  • H. Koschine
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


It is well established that isoproterenol stimulates cardiac ß-adrenergic receptors1 and induces a positive inotropic effect which is Ca++-mediated and which eventually leads to a decline of the ATP concentration2,3, isoproterenol also induces an enhancement of the biosynthesis of myocardial adenine nucleotides (AN) in rat hearts3, which, however, is not of such a magnitude that it has an appreciable effect on the diminution of the ATP concentration. This seems to be mainly due to the fact that cardiac AN biosynthesis is limited by the available pool of 5-phosphoribosyl-1-pyrophosphate (PRPP) which is supplied by the hexose monophosphate shunt4,5. Ribose which bypasses the hexose monophosphate shunt has been shown to overcome this limitation thus leading to an elevation of the available PRPP pool and to an increase of the biosynthesis of AN in rat hearts in vivo4. On the basis of these findings studies were performed to examine whether ribose may affect the isoproterenol-induced diminution of cardiac ATP concentration when applied as continuous i.v. infusion over a longer period of time.


Adenine Nucleotide Positive Inotropic Effect Ehrlich Ascites Tumor Cell Hexose Monophosphate Purine Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    G. A. Robison, R. W. Butcher, I. Øye, H. E. Morgan and E. W. Sutherland, The effect of epinephrine on adenosine 3′:5′-phosphate levels in the isolated perfused rat heart, Mol. Pharmacol. 1:168 (1965)PubMedGoogle Scholar
  2. 2.
    A. Fleckenstein, Specific inhibitors and promoters of Ca++ action, in “Calcium and the Heart”, P. Harris and L. Opie, eds., pp. 135, Academic Press, London and New York (1971)Google Scholar
  3. 3.
    H.-G. Zimmer and E. Gerlach, Effect of ß-adrenergic stimulation on myocardial adenine nucleotide metabolism, Circulation Res. 35:536 (1974)PubMedCrossRefGoogle Scholar
  4. 4.
    H.-G. Zimmer and E. Gerlach, Stimulation of myocardial adenine nucleotide biosynthesis by pentoses and pentitols, Pflügers Arch. 376:223 (1978)PubMedCrossRefGoogle Scholar
  5. 5.
    H.-G. Zimmer and H. Ibel, Effects of isoproterenol and dopamine on the myocardial hexose monophosphate shunt, Experientia 35:510 (1979)PubMedCrossRefGoogle Scholar
  6. 6.
    E. Gerlach, B. Deuticke, R. H. Dreisbach and C. W. Rosarius, Zum Verhalten von Nucleotiden und ihren dephosphorylierten Abbauprodukten in der Niere bei Ischämie und kurzzeitiger postischämischer Wiederdurchblutung, Pflügers Arch. 278:296 (1963)CrossRefGoogle Scholar
  7. 7.
    H.-G. Zimmer, C. Trendelenburg, H. Kammermeier and E. Gerlach, De novo synthesis of myocardial adenine nucleotides in the rat: Acceleration during recovery from oxygen deficiency. Circulation Res. 32:635 (1973)PubMedCrossRefGoogle Scholar
  8. 8.
    J. M. Buchanan and M. P. Schulman, Biosynthesis of the purines. III. Reactions of formate and inosinic acid and an effect of the citrovorum factor. J. Biol. Chem. 202:241 (1953)PubMedGoogle Scholar
  9. 9.
    J. F. Henderson and G. A. LePage, Purine biosynthesis de novo in mouse tissues and a mouse tumor. J. Biol. Chem. 234:2364 (1959)PubMedGoogle Scholar
  10. 10.
    T. Sato, H. Kochi, N. Sato and G. Kikuchi, Glycine metabolism by rat liver mitochondria. III. The glycine cleavage and the exchange of carboxyl carbon of glycine with bicarbonate. J. Biol. Chem. 65:77 (1969)Google Scholar
  11. 11.
    T. Yoshida and G. Kikuchi, Significance of the glycine cleavage system in glycine and serine catabolism in avian liver. Arch. Biochem. Biophys. 145:658 (1971)PubMedCrossRefGoogle Scholar
  12. 12.
    J. F. Henderson and M. K. Y. Khoo, In the mechanism of feedback inhibition of purine biosynthesis de novo in Ehrlich ascites tumor cells in vitro. J. Biol. Chem. 240:3104 (1965)PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • H.-G. Zimmer
    • 1
  • H. Ibel
    • 1
  • G. Steinkopff
    • 1
  • H. Koschine
    • 1
  1. 1.Physiologisches InstitutUniversität MünchenMünchen 2Germany

Personalised recommendations