Addition Products of Uric Acid and Formaldehyde
In order to calibrate colorimetric determinations of serum and urine uric acid, standard dilutions are routinely prepared from a stock solution of uric acid in 2% Formalin. We made a similar solution by dissolving 100 mg of uric acid in 100 ml of 1% formaldehyde in water and applied it to a Polyacrylamide column (BIO GEL P-2) monitored spectrophotometrically at 280 nm. Uric acid, dissolved in water or in lithium carbonate solution, characteristically adsorbs to the column matrix and ultimately elutes as a single peak after 33 hours (1). In contrast, the solution of uric acid with formaldehyde unexpectedly resulted in three distinct elution peaks. A small peak was seen with the characteristic slow mobility of free uric acid, but two much larger peaks, designated F2 and F1, were eluted earlier after 19 and 25 hours. This simple chromatographic technique thus demonstrated the presence of two new reaction products and isolated them for further characterization.
KeywordsUric Acid Adduct Formation Column Fraction Free Formaldehyde Urine Uric Acid
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