A Rapid Screening Method for Inborn Errors of Purine and Pyrimidine Metabolism Using Isotachophoresis

  • H. A. Simmonds
  • A. Sahota
  • R. Payne
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)

Abstract

Isotachophoresis has recently been used in the analysis of purine nucleotides and amino acids1,2. The speed, sensitivity and high resolving power of this relatively new technique suggested it might have potential for the separation of purines and pyrimidines in biological fluids.

Keywords

Adenosine Adenine Pyrimidine Purine Nucleoside 

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References

  1. 1.
    Gower, D. C. and Woledge, R. C., 1977, The use of isotacho-phoresis for the analysis of nuscle extract. Sci. Tools, 24:17.Google Scholar
  2. 2.
    Everaerts, F. M. and Van der Put, A. J. M., 1970, Isotacho-phoresis: The separation of amino acids. J. Chromatog., 52:415.CrossRefGoogle Scholar
  3. 3.
    Sahota, A., Simmonds, H. A. and Payne, R. H., 1979, Separation of urinary purines and pyrimidines by isotachophoresis: Usefulness in screening for inborn errors of purine and pyrimidine metabolism. J. Pharmacol. Methods, 2:303.CrossRefGoogle Scholar
  4. 4.
    Simmonds, H. A., Sahota, A., Potter, C. F. and Cameron, J. S. 1978, Purine metabolism and immunodeficiency: Urinary purine excretion as a diagnostic screening test in adenosine deaminase and purine nucleoside Phosphorylase deficiency. Clin. Sci. Mol. Med., 54:579.PubMedGoogle Scholar
  5. 5.
    Hoffee, P. A. and Jones, M. E. (eds), 1978, Methods in Enzymology: Purine and Pyrimidine Nucleotide Metabolism, Academic Press, New York.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • H. A. Simmonds
    • 1
    • 2
  • A. Sahota
    • 1
    • 2
  • R. Payne
    • 1
    • 2
  1. 1.Clinical Science LaboratoriesGuy’s HospitalLondonUK
  2. 2.LKB InstrumentsSelsdon, South CroydonUK

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