Urate-Binding Proteins in Plasma Studied by Affinity Chromatography
The existence in humans of one or more plasma proteins, which specifically bind uric acid (UA) is still discussed. There is no agreement on the nature of the binding protein, identified as the serum albumin1, or as a specific α1-α2-globulin2, nor on the extent of the bound quote. Most investigations have been till now performed by ultrafiltration1 or column chromatography2, but the specificity and the sensitivity of both are rather unsatisfactory; for example, recovery is lesser than 30% in the later technique3. On the other hand, the existence itself of a significant urate binding to human plasma proteins has been denied even recently, by means of continuous ultrafiltration and equilibrium dialysis4.
KeywordsUric Acid Affinity Chromatography Borate Buffer Specific Ligand Cyanogen Bromide
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