Determinants of 5-Phosphoribosyl-1-Pyrophosphate (PRPP) Synthesis in Human Fibroblasts

  • Kari O. Raivio
  • Cheri Lazar
  • Henry Krumholz
  • Michael A. Becker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)

Abstract

The synthesis of PRPP in the intact cell depends on the in vivo activity of PRPP synthetase and on the availability of its substrates, ribose-5-phosphate (R5P) and ATP. We have studied the regulation of PRPP synthesis in cultured human fibroblasts, particularly the role of intracellular inorganic phosphate (Pi) as an activator of the enzyme and the supply of R5P for the reaction. The potential pathways of R5P generation include the oxidative and nonoxidative branches of the pentose phosphate shunt (PPS), reutilization of ribose-1-phosphate released in nucleotide catabolism by nucleoside Phosphorylase, and phosphorylation of free ribose.

Keywords

Adenine Purine Nucleoside Pentose Ribose 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    J. Katz and H. G. Wood, J. Biol. Chem. 238:517 (1963).PubMedGoogle Scholar
  2. 2.
    M. A. Becker, Biochim. Biophys. Acta 435:132 (1976).PubMedCrossRefGoogle Scholar
  3. 3.
    P. S. Chen Jr., T. Y. Turibara and H. Warner, Anal. Chem. 28: 1756 (1956).CrossRefGoogle Scholar
  4. 4.
    I. H. Fox and W. N. Kelley, J. Biol. Chem. 246:5739 (1971).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Kari O. Raivio
    • 1
    • 2
  • Cheri Lazar
    • 1
    • 2
  • Henry Krumholz
    • 1
    • 2
  • Michael A. Becker
    • 1
    • 2
  1. 1.Children’s HospitalUniversity of HelsinkiFinland
  2. 2.Veterans Administration HospitalSan DiegoUSA

Personalised recommendations