Activity of Ecto-5′-Nucleotidase in Lymphoblastoid Cell Lines Derived from Carriers of Congenital X-Linked Agammaglobulinemia

  • Linda F. Thompson
  • Gerry R. Boss
  • Annie Bianchino
  • J. Edwin Seegmiller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


Patients with congenital X-linked agammaglobulinemia (CAG)* have 1/2 to 1/3 the normal activity of ecto-5′-nucleotidase (ecto-5′-NT) in their peripheral blood mononuclear cells (1,2). Since peripheral B cells have at least 3 times more ecto-5′-NT activity than peripheral T cells (2,3) this deficiency can be largely explained by the absence of circulating B cells in these patients (4). In an attempt to develop a biochemical test for detection of carriers for CAG, ecto-5′-NT was measured in peripheral B cells and in lymphoblastoid (B) cell lines established from mothers and sisters of patients with CAG.


Lymphoblastoid Cell Line Female Relative Nucleotidase Activity Peripheral Blood Mono Rosetting Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



congenital X-linked agammaglobulinemia




peripheral blood mononuclear cells


surface immunoglobulin


Epstein-Barr virus


N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid


inosine 5′-monophosphate


α, β-methylene adenosine 5′-diphosphate


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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Linda F. Thompson
    • 1
  • Gerry R. Boss
    • 1
  • Annie Bianchino
    • 1
  • J. Edwin Seegmiller
    • 1
  1. 1.Department of MedicineUniversity of CaliforniaSan Diego, La JollaUSA

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