Human 5′-nucleotidase. Properties and Characterization of the Enzyme from Placenta, Lymphocytes and Lymphoblastoid Cells in Culture

  • Wolf Gutensohn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


Significantly decreased activities of the ectoenzyme 5′-nucleotidase (EC in peripheral lymphocytes of patients with primary hypogammaglobulinemia have been described. There is conflicting evidence in the literature whether this concerns the X-linked type of the disease or rather the socalled “adult onset” hypogammaglobulinemia1,2,3 . Nevertheless, it seemed interesting to study the properties of human 5′-nucleotidase (5′-N) more closely. For reasons of availability of starting material the following approach was chosen: First the placental 5′-N was purified and characterized followed by an extensive comparison of the properties of this enzyme with 5′-N from human lymphocytes and lymphoblastoid cells


Lymphoblastoid Cell Line Peripheral Lymphocyte Alkaline Phospha Isotonic Condition Human Lymphoblastoid Cell Line 


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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Wolf Gutensohn
    • 1
  1. 1.Arbeitsgruppe Biochemische HumangenetikInstitut für Anthropologie und Humangenetik der UniversitätMünchen 2Fed. Rep. Germany

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