Human 5′-nucleotidase. Properties and Characterization of the Enzyme from Placenta, Lymphocytes and Lymphoblastoid Cells in Culture
Significantly decreased activities of the ectoenzyme 5′-nucleotidase (EC 18.104.22.168) in peripheral lymphocytes of patients with primary hypogammaglobulinemia have been described. There is conflicting evidence in the literature whether this concerns the X-linked type of the disease or rather the socalled “adult onset” hypogammaglobulinemia1,2,3 . Nevertheless, it seemed interesting to study the properties of human 5′-nucleotidase (5′-N) more closely. For reasons of availability of starting material the following approach was chosen: First the placental 5′-N was purified and characterized followed by an extensive comparison of the properties of this enzyme with 5′-N from human lymphocytes and lymphoblastoid cells
KeywordsLymphoblastoid Cell Line Peripheral Lymphocyte Alkaline Phospha Isotonic Condition Human Lymphoblastoid Cell Line
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- 4.W. Gutensohn, Fractionation of human plasmamembrane ectoenzymes by ionexchange-, lectin-specific and substrate-specific affinity-chromatography, in: “Proceedings of the 27th Colloquium: Protides of the Biological Fluids”, (Brussels, 1979), Pergamon Press, Oxford (1979), in press.Google Scholar