Possible Role for 5′-Nucleotidase in Deoxyadenosine Selective Toxicity to Cultured Human Lymphoblasts
Adenosine deaminase deficiency and purine nucleoside Phosphorylase deficiency are relatively specific for causing a disorder of immune function. Several in vitro observations provide clues concerning the basis for this specificity. The addition of deoxyadenosine reduces the response of peripheral blood lymphocytes to mitogen stimulation when adenosine deaminase is inhibi ted1,2. The combination of deoxyadenosine and adenosine deaminase inhibition is also cytotoxic to T-lymphoblasts but not B-lymphoblasts3–5. Deoxyadenosine mediated cytoxicity in T-lymphoblasts is accompanied by increased concentrations of dATP.
KeywordsToxicity Adenosine Purine Nucleoside Triphosphate
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- 8.N. L. Edwards, E. W. Gelfand, L. Burk, H. M. Dosch, and I. H. Fox, Distribution of 5′-nucleotidase in human lymphoid tissue. Proc. Natl. Acad. Sci. USA 76:(In Press) (1979).Google Scholar