Increases in Purine Excretion and Rate of Synthesis by Drugs Inhibiting IMP Dehydrogenase or Adenylosuccinate Synthetase Activities
Hershfield reported an assay system devoid of hypoxanthine for evaluating the rate of purine synthesis de novo based on rates of [14C]-formate incorporation into intracellular purine components of lymphoblasts and into purines excreted into the culture medium. Using this system normal and in vitro selected, biochemically-characterized HPRT* deficient lines were found to have similar rates of total purine synthesis; however, these cell lines differed markedly in the excretion of newly-formed purine into the medium (1,2).
KeywordsMycophenolic Acid Purine Nucleoside Purine Nucleoside Phosphorylase Purine Synthesis Inosine Monophosphate
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