Activities of Enzymes of Purine and Pyrimidine Metabolism in Nine Mycoplasma Species
Cultured fibroblasts are commonly used for studies of purine and pyrimidine metabolism and may be helpful for the prenatal diagnosis of enzyme deficiencies. Mycoplasmas, a common contaminant of cell cultures, are unable to realize “de novo” synthesis of purines and pyrimidines. It is necessary to add to the medium precursors such as free bases (guanine, uracil, thymine) or corresponding nucleosides or nucleotides in order to obtain a good growth in vitro. The study of the best medium composition shows that mycoplasmas possess active pathways of nucleotides degradation and bases salvage (1).
KeywordsAdenosine Deaminase Cytidine Deaminase Normal Human Fibroblast Skin Fibroblast Culture Final Protein Concentration
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