Simultaneous Determination of Rates of Purine and Pyrimidine Synthesis in Cultured Human Lymphoblasts and Fibroblasts

  • William H. Huisman
  • Kari O. Raivio
  • Michael A. Becker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


Measurement of rates of incorporation of radiolabeled precursors into pathway intermediates and end products has commonly been employed in the study of rates of specific biosynthetic processes in intact cells. We report here the development and validation of an isotopic method which exploits the shared requirement of the pathways of both purine and pyrimidine nucleotide synthesis de novo for one molecule of CO2 per base moiety to provide simultaneous estimates of rates of these pathways in cultured human lymphoblasts and fibroblasts.


Purine Base Pathway Intermediate Pyrimidine Nucleoside Label Incorporation Pyrimidine Synthesis 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • William H. Huisman
    • 1
    • 2
  • Kari O. Raivio
    • 1
    • 2
  • Michael A. Becker
    • 1
    • 2
  1. 1.Veterans Administration Medical CenterSan Diego, La JollaUSA
  2. 2.University of CaliforniaSan Diego, La JollaUSA

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