Simultaneous Determination of Rates of Purine and Pyrimidine Synthesis in Cultured Human Lymphoblasts and Fibroblasts
Measurement of rates of incorporation of radiolabeled precursors into pathway intermediates and end products has commonly been employed in the study of rates of specific biosynthetic processes in intact cells. We report here the development and validation of an isotopic method which exploits the shared requirement of the pathways of both purine and pyrimidine nucleotide synthesis de novo for one molecule of CO2 per base moiety to provide simultaneous estimates of rates of these pathways in cultured human lymphoblasts and fibroblasts.
KeywordsPurine Base Pathway Intermediate Pyrimidine Nucleoside Label Incorporation Pyrimidine Synthesis
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- 1.W.H. Huisman, K.O. Raivio, and M.A. Becker, J.Biol.Chem. In Press.Google Scholar
- 2.T. Hovi, A.C. Allison, K.O. Raivio, and A. Vaheri, jm: “Purine and Pyrimidine Metabolism,” K. Elliott and D.W. Fitzsimons, eds., Ciba Foundation Symposium 48 (New series) 225, Elsvier, Amsterdam (1977).Google Scholar
- 3.K. Ito and H. Uchino, J.Biol.Chem. 246:4069 (1971).Google Scholar
- 4.F.F. Snyder, M.S. Hershfield, and J.E. Seegmiller, Adv.Exp. Med.Biol. 76A:30 (1976).Google Scholar
- 7.G. Nuki, J. Lever, and J.E. Seegmiller, Adv.Exp.Med.Biol. 41A:255 (1974).Google Scholar
- 8.W.H. Huisman and M.A. Becker, submitted for publication.Google Scholar
- 9.M.S. Hershfield, E. Spector, and J.E. Seegmiller, Adv.Exp.Med.Biol. 76A:303 (1976).Google Scholar