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Guanase from Human Liver — Purification and Characterisation

  • Rudolf Kuzmits
  • Heinrich Stemberger
  • Mathias M. Müller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)

Abstract

Guanine aminohydrolase (E. C. 3.5.4.3.) plays an important role in the catabolism of purines, catalyzing the formation of xanthine from guanine.

Keywords

Human Liver Ammonium Sulphate Ammonium Sulphate Fractionation Sulphate Satur Ammonium Sulphate Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    van Bennekom, C.A., van Laarhoven, J.P., de Bruyn, C.H.M.M. and Oei, T.L.: A simple and sensitive radiochemical assay for plasma guanase. J.Clin.Chem.Clin.Biochem. 16: 245–248 (1978).PubMedGoogle Scholar
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    Svensson, H.: Isoelectric fractionation, analysis and characterization of ampholytes in natural pH-gradients. III. Description of apparatus for electrolysis in columns stabilized by density gradients and direct determination of isoelectric points. Arch. Biochem. Biophys. Suppl. 1: 132–138 (1962)Google Scholar
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    Sree Kumar, K., Sitaramayya, A. and Krishnan, P.S.: Guanine deaminase in rat liver and mouse liver and brain. Biochem. J. 128: 1079–1088 (1972).Google Scholar
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    Farkas, W.R. and Singh, R.D.: Guanine aminohydrolase in rat and mouse red cells: A potent inhibitor of guanylation of tRNA. Biochim. Biophys. Acta 377: 166–173 (1975).PubMedCrossRefGoogle Scholar
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    Ureta, T.: In Current Topics in Cellular Regulation, (Horecker, B.L. and Stadtmann, E.R.), Vol 3., pp 233–258, Academic Press, New York (1978).Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Rudolf Kuzmits
    • 1
  • Heinrich Stemberger
    • 1
  • Mathias M. Müller
    • 1
  1. 1.Dept. of Medical Chemistry, Institute for Specific Prophylaxis and Tropical Medicine, 2nd Dept. of MedicineUniversity of ViennaAustria

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