Adenosine and Deoxyadenosine Kinase from Rat Liver

  • Nobuaki Ogasawara
  • Yasukazu Yamada
  • Haruko Goto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


Recent studies demonstrated that dATP concentration was markedly increased in erythrocytes from patient with adenosine deaminase deficiency1,2 . Deoxyadenosine is toxic to T lymphoblast in the presence of an inhibitor of adenosine deaminase. By the addition of deoxyadenosine, dATP accumulated in the cell and the level of dATP well corelated with the cytotoxicity4,5.


Kinase Activity Pyruvate Kinase Adenosine Deaminase Severe Combine Immunodeficiency NADH Oxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. Cohen, R. Hirschhorn, S. D. Horowitz, A. Rubinstein, S. H. Polmar, R. Hong and D. W. Martin Jr., Deoxyadenosine triphosphate as a potentially toxic metabolite in adenosine deaminase deficiency, Proc. Natl. Acad. Sci. USA, 75:472 (1978).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Donofrio, M. S. Coleman, J. J. Hutton, A. Daoud, B. Lampkin and J. Dyminski, Overproduction of adenine deoxynucleosides and deoxynucleotides in adenosine deaminase deficiency with severe combined immunodeficiency disease, J. Clin. Invest., 62:884 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    B. Ullman, L. J. Gudas, A. Cohen and D. W. Martin, Jr., Deoxyadenosine metabolism and cytotoxicity in cultured mouse T lymphoma cells: a model for immunodeficiency, Cell, 14:365 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    B. S. Mitchell, E. Mejias, P. E. Daddona and W. N. Kelley, Purinogenic immunodeficiency diseases: selective toxicity of deoxyribonucleosides to T cells, Proc. Natl. Acad. Sci. USA, 75:5011 (1978).PubMedCrossRefGoogle Scholar
  5. 5.
    D. G. Streeter, L. N. Simon, R. K. Robins and J. P. Miller, The phosphorylation of ribavirin by deoxyadenosine kinase from rat liver. Differentiation between adenosine and deoxyadenosine kinase, Biochemistry, 13:4543 (1974).PubMedCrossRefGoogle Scholar
  6. 6.
    D. L. Weiler, A. Heaney and R. E. Sjogren, A simple apparatus and procedure for electrofocusing experiments: pI of lactate dehydrogenase and isocitrate lyase, Biochim. Biophys. Acta, 168:576 (1968).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Nobuaki Ogasawara
    • 1
  • Yasukazu Yamada
    • 1
  • Haruko Goto
    • 1
  1. 1.Department of BiochemistryInstitute for Developmental ResearchAichi Prefectural Colony, Kasugai, AichiJapan

Personalised recommendations