Structural Studies of Human Adenine Phosphoribosyltransferase Purified by Affinity Chromatography
Adenine phosphoribosyltransferase (APRT) catalyzes the condensation of 5-phosphoribosyl-1-pyrophosphate (PP-ribose-P) with adenine to yield adenosine 5’-monophosphate and PPi. Interest in the human enzyme has been stimulated by the findings of elevated adenine phosphoribosyltransferase activity in erythrocytes from patients with the Lesch-Nyhan syndrome1 and by the inherited deficiency of the enzyme described in a number of families.2,3,4,5 In order to better understand the nature of these alterations of adenine phosphoribosyltransferase activity at the molecular level, it is necessary to define the nature of the normal enzyme. The normal enzyme has previously been purified 33,000-fold from human erythrocytes.6 The procedure, however, is lengthy and laborious. In this report we describe a more efficient purification procedure for adenine phosphoribosyltransferase. Some of the characteristics of the highly purified enzyme, not previously reported, are described.
KeywordsAmino Acid Composition Guanidine Hydrochloride Hydrolysis Time Performic Acid Normal Enzyme
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