Chemical Modification of Hypoxanthine-Phosphoribosyltransferase and Its Protection by Substrates and Products
The importance of hypoxanthine-phosphoribosyltransferase (HPRT) to human biochemical genetics need not be stressed in this context. Although in some cases it has not been clear whether deficiencies of this enzyme are due to a structural mutation this seems to be well established in others. Hence a detailed knowledge of the structure and especially of essential amino acid residues seems desirable. Induced by a similar study on quinolinate-phosphoribosyltransferase1 and parallel to our experiments on specific irreversible inhibition chemical modification of HPRT was investigated.
KeywordsMethylene Blue Sulfonic Acid Quinolinic Acid Purine Nucleoside Structural Mutation
Unable to display preview. Download preview PDF.