The Effect of Phosphoribosylpyrophosphate on Stability and Configuration of Hypoxanthineguaninephosphoribosyltransferase and Adeninephosphoribosyltransferase from Human Erythrocytes

  • W. Gröbner
  • N. Zöllner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)


5-Phosphoribosylpyrophosphate (PRPP) is a substrate of hypoxanthineguaninephosphoribosyltransferase (HGPRT) and adeninephosphoribosyltransferase (APRT). It stabilizes these purine phosphoribosyltransferases against thermal inactivation in vitro. Using sucrose density gradient ultracentrifugation we investigated whether this stabilisation is associated with a change in the configuration of these enzymes.


Buffy Coat Thermal Inactivation Large Form Purine Metabolism Sedimentation Coefficient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arnold, W. N., Kelley, W. N., 1971, J. Biol. Chem., 246:7398.PubMedGoogle Scholar
  2. Holden, J. A., Kelley, W. N., 1978, J. Biol. Chem., 253:4459.PubMedGoogle Scholar
  3. Lowry, O. H., Rosebrough, N. J., Parr, A. L., Randall, R. J., 1951, J. Biol. Chem., 193:265.PubMedGoogle Scholar
  4. McCarty, K. S., Staffort, D., Brown, O., 1968, Anal. Biochem., 24:314.PubMedCrossRefGoogle Scholar
  5. Natsumeda, Y., Yoshino, M., Tsushima, K., 1977, Biochim. Biophys. Acta, 483:63.PubMedCrossRefGoogle Scholar
  6. Olsen, A. S., Milman, G., 1977, Biochem., 16:2501.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • W. Gröbner
    • 1
  • N. Zöllner
    • 1
  1. 1.Medical PoliclinicUniversity of MunichMunichGermany

Personalised recommendations