The Effect of Phosphoribosylpyrophosphate on Stability and Configuration of Hypoxanthineguaninephosphoribosyltransferase and Adeninephosphoribosyltransferase from Human Erythrocytes
5-Phosphoribosylpyrophosphate (PRPP) is a substrate of hypoxanthineguaninephosphoribosyltransferase (HGPRT) and adeninephosphoribosyltransferase (APRT). It stabilizes these purine phosphoribosyltransferases against thermal inactivation in vitro. Using sucrose density gradient ultracentrifugation we investigated whether this stabilisation is associated with a change in the configuration of these enzymes.
KeywordsBuffy Coat Thermal Inactivation Large Form Purine Metabolism Sedimentation Coefficient
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