Abstract
In mammals, the enzyme adenine phosphoribosyl transferase (APRT; AMP: pyrophosphate phosphoribosyl transferase; E.C. 2·42·7) is the only known mechanism by which dietary adenine can be utilized1. In humans with reduced or absent APRT activity, renal stones containing substantial amounts of 2,8-dioxyadenine can occur1. However, other APRT deficient individuals do not suffer urolithiasis and appear to be asymptomatic. Thus, the role of APRT, and, indeed, the role of adenine, in the metabolism of mammals is unclear.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
A.W. Murray, D.C. Elliot, and M.R. Atkinson, Nucleotide biosynthesis from preformed purines in mammalian cells: regulatory mechanisms and biological significance, Prog. Nuc. Acid Res. Mol. Biol. 10:87 (1970).
K.J. Van Acker, J.A. Simmonds, C. Potter and J.S. Cameron, Complete deficiency of adenine phosphoribosyltransferase report of a family, New Engl. J. Med. 297:127–132 (1977).
S.K. Srivastava and E. Bentler, Purification and kinetic studies of adenine phosphoribosyltransferase from human erythrocytes, Arch. Biochem. Biophys. 142:426–434 (1971).
R.E.A. Gadd and J.F. Henderson, Studies of the binding of phosphoribosylpyrophosphate to adenine phosphoribosyltransferase, J. Biol. Chem. 245:2979–2984 (1970).
C.B. Thomas, W.J. Arnold, and W.N. Kelley, Human adenine phosphoribosyltransferase purification, subunit structure, and substrate specificity, J. Biol. Chem. 248:2529–2535, (1973).
W. Mowbray, B. Watson, H. Harris, A search for electrophoretic variants of human adenine phosphoribosyltransferase, Ann. Human Genetics 36:153 (1972).
J.G. Kenimer, L.G. Young and D.P. Groth, Purification and proprties of rat liver adenine phosphoribosyltransferase, Biochim. Biophys. Acta 384:87–101 (1975).
H.V. Hershey and M.W. Taylor, Purification of adenine phosphoribosyltransf erase by affinity chromatography, Prep. BioChem. 8(6):453 (1978).
K.R. Held, B. Kahan, and R. DeMars, Adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase immunoprecipitation reactions in human-mouse and human-hamster cell hybrids, Humangenetik 30:23–34 (1975).
K. Weber and Osborn, The reliability of molecular weight determinations by dodecvl sulfate-polyacrylamide gel electrophoresis, J. Biol. Chem. 244:4406–4412, (1969).
P. Maisudaira, and D.R. Burgess, SDS microslab linear gradient Polyacrylamide gel electrophoresis, Anal. Biochem. 87:386–396, (1978).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Taylor, M.W., Hershey, H.V. (1980). Purification and Characterization of Mammalian Adenine Phosphoribosyltransferases. In: Rapado, A., Watts, R.W.E., De Bruyn, C.H.M.M. (eds) Purine Metabolism in Man—III. Advances in Experimental Medicine and Biology, vol 122B. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8559-2_19
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8559-2_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8561-5
Online ISBN: 978-1-4684-8559-2
eBook Packages: Springer Book Archive