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Effect of Actinomycin D on in vivo Purine Biosynthesis in Hamster Cells

  • Milton W. Taylor
  • Kailash C. Gupta
  • Ludmila Zawistowich
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 122B)

Abstract

In the course of studying the possible induction of enzymes associated with purine biosynthesis in the Chinese Hamster Cell line, V79, we wished to ascertain whether any newly synthesized short-lived mRNA species, or proteins were required for regulation of the de novo pathway. This was done by measuring in vivo purine biosynthesis in the presence of an inhibitor of RNA synthesis (Actinomycin D), and inhibitors of protein synthesis (cycloheximide and puromycin). Although we could not, at this stage, find evidence of a need for new mRNA or proteins, a direct effect of both actinomycin D and puromycin on de novo purine biosynthesis was noted.

Keywords

mRNA Synthesis Purine Biosynthesis Subconfluent Monolayer Characteristic Dose Response Chinese Hamster Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Feldman, R.F., and Taylor, M.W., 1974, Purine mutants of mammalian cell lines. I Mutant lacking FGAR amidotransferase activity, Biochem. Genetics, 12:393.CrossRefGoogle Scholar
  2. Hershfield, M.S., and Seegmiller, J.E., 1977, Regulation of de novo purine synthesis in human lymphoblasts, Jour. Biol. Chem., 252:6002.Google Scholar
  3. Perry, R.P., and Kelley, D.E., 1979, Inhibition of RNA synthesis by actinomycin D: Characteristic dose response curve of different RNA species, J. Cell Physiol., 76:127.CrossRefGoogle Scholar
  4. Taylor, M.W., Tokito, M.K., Gupta, K.C., and Pipkorn, J., Regulation of purine de novo biosynthesis in Chinese hamster ovary cells: effect of glutamine starvation, Bioch. Acta., 517:1.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Milton W. Taylor
    • 1
  • Kailash C. Gupta
    • 1
  • Ludmila Zawistowich
    • 1
  1. 1.Department of BiologyIndiana UniversityBloomingtonUSA

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