De Novo Purine Synthesis in Cultured Human Fibroblasts
In mammalian cells, purine nucleotides may be formed via (a) de novo synthesis and (b) salvage pathways. Both these pathways utilise PRPP and the concentration of this substrate is thought to be important in regulating the rate of purine synthesis (1, 2). Individuals with a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT), have increased production of uric acid (3, 4). Lymphocyte and fibroblast cultures of HPRT-deficient (HPRT-) cells have been reported as having accelerated rates of purine de novo synthesis associated with elevated concentrations of PRPP (5, 6).
KeywordsSalvage Pathway Purine Base Hypoxanthine Phosphoribosyltransferase Purine Synthesis Dialyse Serum
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