The Paraventriculo-Infundibular Corticotropin Releasing Factor (CRF)-System II. Functional Aspects

  • István Lengvári
  • Magdolna Kovács
  • Zsolt Liposits
  • Sándor Vigh
  • Béla Flerkó
Part of the Biochemical Endocrinology book series (BIOEND)


Recent isolation, characterisation and synthesis of CRF, a 41-residue polypeptide (Vale et al., 1981) made it possible to produce specific antibodies against it, which in turn resulted in immunohistochemical detection of CRF containing structures within the central nervous system (Merchenthaler et al., 1982: Swanson et al., 1983). One of the main characteristics of the central CRF system is that CRF containing cell bodies are found in diverse parts of the central nervous system. CRF containing cell bodies are demonstrated in several limbic areas, besides the hypothalamus (Liposits et al., 1983a: Merchenthaler et al., 1983), and even a few cells are scattered in the cerebral cortex (Merchenthaler, 1984: Fig. 1). It is evident that all of these cell groups are not directly responsible for the ACTH release of the anterior pituitary, as some of the areas containing CRF-immunopositive cells do not have classically demonstrated connection with the median eminence. In this presentation we try to answer two questions: (1) which CRF cell group is responsible for the pituitary ACTH release; and (2) what is the role of the other CRF cell groups.


Corticotropin Release Factor Paraventricular Nucleus Median Eminence Plasma Corticosterone ACTH Secretion 
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  1. Ambach, G., and Palkovits, M., 1974, Blood supply of the rat hypothalamus. II. Nucleus paraventricularis, Acta Morph. Acad. Sci, hung., 22:311.Google Scholar
  2. Cruce, A. F., 1975, An autoradiographic study of the projections of the mammillothalamic tract in the rat, Brain Res., 85:211.PubMedCrossRefGoogle Scholar
  3. Cruce, A. F., 1976, An autoradiographic study of the descending connections of the mammillary nuclei of the rat, J. Comp, neurol., 176: 631.CrossRefGoogle Scholar
  4. Dunn, J., and Critchlow, V., 1972, Electrically stimulated ACTH release in pharmacologically blocked rats, Endocrinology, 93:835.CrossRefGoogle Scholar
  5. De Groot, J., and Harris, G. W., 1950, Hypothalamic control of the anterior pituitary gland and blood lymphocytes, J. Physiol., 111:335.Google Scholar
  6. Fellman, D., Bugnon, C., and Gouget, A., 1982, Immunocytochemical demonstration of corticoliberin-like immunoreactivity (CLI) in neurons of the rat amygdala central nucleus (ACN), Neurosci. Lett., 34:253.Google Scholar
  7. Fishman, A. J., and Moldow, R. L., 1982, Distribution of CRF-like immunoreactivity in the rabbit. Peptides 3:842.Google Scholar
  8. Halász, B., and Pupp, L., 1965, Hormone secretion of the anterior pituitary gland after physical interruption of all nervous pathways to the hypophyseotrophic area, Endocrinology 77:553.PubMedCrossRefGoogle Scholar
  9. Halász, B., Slusher, M., and Gorski, R. A., 1967, Adrenocorticotrophic hormone secretion in rats after partial or total deafferentation of the medial basal hypothalamus, Neuroendocrinology 2:43.CrossRefGoogle Scholar
  10. Kovács, M., Lengvári, I., Liposits, Zs., Vigh, S., Schally, A. V., and Flerkó, B., 1985, CRF-immunoreactive neurons in the mammillary body of the rat, in press.Google Scholar
  11. Lengvári, I., Kovács, M., and Szelier, M., 1983, The effect of electrochemical stimulation of paraventricular nuclei on plasma corticosterone, Neuroendocrinol. Lett. 5:207.Google Scholar
  12. Lengvári, I., Kovács, M., Liposits, Zs., and Szelier, M., 1984, The effect of stimulation of mammillary body on plasma corticosterone in rat, Neuroendocrinol. Lett. 6: 285.Google Scholar
  13. Lengvári, I., Kovács, M., Liposits, Zs., and Szelier, M., 1985a, The lack of effect of electrochemical stimulation of the hypothalamus and median eminence on the plasma corticosterone level after lesion of the paraventricular nuclei, Exp. Clin. Endocrinol. in press.Google Scholar
  14. Lengvári, I., Liposits, Zs., Vigh, S, Schally, A.V., and Flerkó, B., 1985b, Re-evaluation of “frontal deafferentation studies on the regulation of ACTH secretion in the light of CRF immunohistology, Endocrinol. experiment, in press.Google Scholar
  15. Liposits, Zs., Göres, T., Sétáló, Gy., Lengvári, I., Flerkó, B., Vigh, S., and Schally, A.V., 1983a, Ultrastructural characteristics of immunolabelied, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain. Cell Tiss. Res. 229:101.Google Scholar
  16. Liposits, Zs., Lengvári, I., Vigh, S., Schally, A. V., and Flerkó, B., 1983b, Immunohistological detection of degenerating CRF-immunoreactive nerve fibers in the median eminence after lesion of paraventricular nucleus of the rat. A light and electronmicroscopic study, Peptides 4:941.Google Scholar
  17. Makara, G. B., Stark, E., Rappay, Gy., Kárteszi, M., and Palkovits, M., 1979, Changes in corticotropin releasing factor of the stalk median eminence in rats with various cuts around the medial basal hypothalamus, J. Endocrinol. 83:165.PubMedCrossRefGoogle Scholar
  18. Merchenthaler, I., 1984, Corticotropin releasing factor (CRF)-like immunoreactivity in the rat central nervous system. Extrahypothalamic distribution. Peptides 5 (Suppl. 1):53.PubMedCrossRefGoogle Scholar
  19. Merchenthaler, I., Vigh, S., Petrusz, P., and Schally, A. V., 1982, Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat brain, Am. J. Anat. 165:385.Google Scholar
  20. Merchenthaler, I., Vigh, S., Petrusz, P., and Schally, A. V., 1983, The paraventriculo-infundibular corticotropin releasing factor (CRF)-pathway as revealed by immunochemistry in longterm hypophysectomized or adrenalectomized rats, Regul. Pept. 5:295.Google Scholar
  21. Merchenthaler, I., Vigh, S., Schally, A.V., Stumpf, W. E., and Arimura, A., 1984, Immunocytochemical localization of corticotropin releasing factor (CRF)-like immunoreactivity in the thalamus of the rat, Brain Res, in press.Google Scholar
  22. Moll, J., 1960, Localization of hypothalamic lesions inhibiting adrenal weight maintenance in the rat, Acta Endocrinol. 34:19.Google Scholar
  23. Palka, Y., Coyer, D., and Critchlow, V., 1969, Effects of isolation of medial basal hypothalamus on pituitary-adrenal and pituitary-ovarian functions, Neuroendocrinology 5:333.PubMedCrossRefGoogle Scholar
  24. Porter, R. W., 1952, Alterations in electrical activity of the hypothalamus induced by stress stimuli. Am. J. Physiol. 169:629.Google Scholar
  25. Porter, R. W., 1953, Hypothalamic involvement in the pituitary-adrenocortical response to stress stimuli. Am. J. Physiol. 172:515.Google Scholar
  26. Sétáló, G., Flerkó, B., Arimura, A., and Schally, A. V., 1978, Brain cells as producers of releasing and inhibiting hormones, Int. Rev. Cytol. Suppl. 7:1.Google Scholar
  27. Shen, C. L., 1983, Efferent projections from the mammillary complex of the guinea pig: An autoradiographic study, Brain Res. Bull. 11:43.Google Scholar
  28. Swanson, L. W., Sawchenko, P. E., Rivier, J., and Vale, W. W., 1983, Organization of ovine corticotropin-releasing immunoreactive cells and fibers in the rat brain: An immunohistochemical study. Neuroendocrinology 36:165.Google Scholar
  29. Vale, W., Spiess, J., Rivier, C., and Rivier, J., 1981, Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and ß-endorphin. Science 213:1394.PubMedCrossRefGoogle Scholar
  30. Vale, W., Rivier, C., Brown, M.R., Spiess, J., Koob, G., Swanson, L., Bilezikjian, L., Bloom, F., and Rivier, J., 1983, Chemical and biological characterization of corticotropin releasing factor, Recent Progr. Hormone Res. 39:245.Google Scholar
  31. Voloshin, L., Joseph, S. A., and Knigge, K. M., 1968, Endocrine function in male rats following complete and partial isolation of the hypothalamo-pituitary unit. Neuroendocrinology 3:387.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • István Lengvári
    • 1
  • Magdolna Kovács
    • 1
  • Zsolt Liposits
    • 1
  • Sándor Vigh
    • 1
  • Béla Flerkó
    • 1
  1. 1.Department of AnatomyUniversity Medical SchoolPécsHungary

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