Fluorescence Recovery after Photobleaching: Lateral Mobility of Lipids and Proteins in Model Membranes and on Single Cell Surfaces

  • Kenneth Jacobson
Part of the Nato Advanced Study Institutes Series book series (NSSA, volume 34)


The fluorescence recovery after photobleaching (FRAP) technique was developed by membrane biophysicists to measure the rate of translational motion of membrane components in the plane of the membrane. This technique has also been named the fluorescence photobleaching recovery or FPR method.


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  1. Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E. and Webb, W.W. (1976) Biophys. J., 16:1055.CrossRefGoogle Scholar
  2. Axelrod, D. (1977) Biophys. J., 18;129.CrossRefGoogle Scholar
  3. Derzko, Z. and Jacobson, K. (1978) Biophys. J., 21:204aGoogle Scholar
  4. Derzko, Z. and Jacobson, K. (1979) Biophys. J., 25:172a.Google Scholar
  5. Edelman, G. (1976) Science 192:218.CrossRefGoogle Scholar
  6. Edidin, M. (1974) Annu. Rev. Biophys. Bioengineer. 1:179.CrossRefGoogle Scholar
  7. Edidin, M., Zagyanski, Y. and Lardner, T. (1976) Science, 191:466.CrossRefGoogle Scholar
  8. Edidin, M. and Wei, T. (1977) J. Cell Biol. 75:475.CrossRefGoogle Scholar
  9. Fahey, P. and Webb, W. (1978) Biochemistry, 17:3046–3053.CrossRefGoogle Scholar
  10. Flanagan, J. and Koch, G. (1978) Nature 273:278.CrossRefGoogle Scholar
  11. Frye, L. and Edidin, M. (1970) J. Cell Sci. 7:319.Google Scholar
  12. Jacobson, K., Wu, E-S. and Poste, G. (1976) Biochim. Biophys. Acta, 433:215.CrossRefGoogle Scholar
  13. Jacobson, K., Derzko, Z., Wu, E-S., Hou, Y. and Poste, G. (1977) J. Supramol. Struct., 5:565.CrossRefGoogle Scholar
  14. Jacobson, K., Hou, Y. and Wojcieszyn, J. (1978) Expt. Cell Res. 116:179.CrossRefGoogle Scholar
  15. Koppel, D., Axelrod, D., Schlessinger, J., Elson, E., and Webb, W. (1976) Biophys. J., 16:1315.CrossRefGoogle Scholar
  16. Lardner, T. and Solomon, N. (1976) J. Theor. Biol. 60:433.CrossRefGoogle Scholar
  17. Liebman, P. and Entine, G. (1974) Science 185:457.CrossRefGoogle Scholar
  18. Lepock, J., Thompson, J., Kruuv, J. and Wallach, D.F.H. (1978) Biochem. Biophys. Res. Comm. 85:344.CrossRefGoogle Scholar
  19. Nicolson, G.L., Poste, G., Ji, T.H. (1977) Cell Surface Reviews (ed. G. Poste and G.L. Nicolson, Vol. 3, p.1, North Holland, Amsterdam).Google Scholar
  20. Nigg, E., Kessler, M. and Cherry, R. (1979) Biochim. Biophys. Acta, 550:328.CrossRefGoogle Scholar
  21. Petit, V. and Edidin, M. (1974) Science, 184:1183.CrossRefGoogle Scholar
  22. Peters, R., Peters, J., Tews, K. and Bahr, W. (1974) Biochim. Biophys. Acta, 367: 282.CrossRefGoogle Scholar
  23. Poo, M.M. and Cone, R. (1974) Nature 247:438.CrossRefGoogle Scholar
  24. Rubenstein, J., Smith, B. and McConnell, H. (1979) Proc. Natl. Acad. Sci. 76:15.CrossRefGoogle Scholar
  25. Saffman, P. and Delbrück, M. (1975) Proc. Natl. Acad. Sci. USA, 723111.CrossRefGoogle Scholar
  26. Schlessinger, J., Koppel, D., Axelrod, D., Jacobson, K., Webb, W., and Elson, E. (1976) Proc. Nat. Acad. Sci. USA, 73:2409.CrossRefGoogle Scholar
  27. Schlessinger, J., Axelrod, D., Koppel, D., Webb, W. and Elson, E. (1977a) Science, 195:307.CrossRefGoogle Scholar
  28. Schlessinger, J., Elson, E. L., Webb, W.W., Yahara, I., Rutishauser, U. and Edelman, G.M. (1977b) Proc. Natl. Acad. Sci. USA, 74:1110.CrossRefGoogle Scholar
  29. Schlessinger, J., Barak, L., Yamada, K., Pastan, I., Webb, W. and Elson, E. (1977c) Proc. Natl. Acad. Sci. USA, 74:2909.CrossRefGoogle Scholar
  30. Schlessinger, J., Schechter, Y., Cuatrecasas, P., Willingham, M. and Pastan, I. (1978) Proc. Nat. Acad. USA, 75:5353.CrossRefGoogle Scholar
  31. Sheetz, M. and Koppel, D. (1979) Proc. Nat. Acad. Sci. USA, 76:3314.CrossRefGoogle Scholar
  32. Sims, P., Waggoner, A., Wang, C-H. and Hoffman, J. (1974) Biochemistry 13:3315–3330.CrossRefGoogle Scholar
  33. Singer, S. and Nicolson, G. (1977) Science, 175:720.CrossRefGoogle Scholar
  34. Smith, B. and McConnell, H. (1978) Proc. Natl. Acad. Sci. USA, 75:2759.CrossRefGoogle Scholar
  35. Smith, B., McConnell, H. and Clark, W. (1979) Biophys. J. 25:62a.CrossRefGoogle Scholar
  36. Smith, L., Smith, B. and McConnell, H. (1979) Biochemistry, 18: 2256.CrossRefGoogle Scholar
  37. Taylor, R., Duffus, W., Raff, M. and DePetris, S. (1971) Nature N.B. 233:225–229.Google Scholar
  38. Vaz, W.L.C., Jacobson, K., Wu, E-S. and Derzko, Z. (1979) Proc. Natl. Acad. Sci. USA, in press.Google Scholar
  39. Wu, E-S., Jacobson, K., Paphadjopoulos, D. (1977) Biochemistry, 16:3926.Google Scholar
  40. Wu, E-S., Jacobson, K., Szoka, F. and Portis, A. (1978) Biochemistry, 17: 5543.CrossRefGoogle Scholar
  41. Zagyansky, Y. and Edidin, M. (1976) Biochim. Biophys. Acta, 433:209.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Kenneth Jacobson
    • 1
  1. 1.Department of Experimental PathologyRoswell Park Memorial InstituteBuffaloUSA

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