Comparison between Paf-Acether Receptors on Intact Washed Human Platelets and Human Endothelial Cells in Culture

  • Ruth Korth
  • M. Hirafuji
  • J. Bidault
  • B. Canton
  • F. Russo-Marie
  • J. Benveniste


Paf-acether (paf, formerly platelet-activating factor) (Benveniste et al., 1972), with the structure 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine (Benveniste et al., 1979; Demopoulos et al., 1979; Blank et al., 1979), is a biologically active phospholipid which is released by several cell types upon stimulation (for review Pinckard et al., 1982; Vargaftig et al., 1981). The mediator activates platelets, polymorphonuclear neutrophils, macrophages and endothelial cells most probably via a receptor-dependent pathway (Valone et al., 1982; Valone and Goetzl 1983; Lambrecht and Parnham, 1986; Korth et al., 1987; Valone, 1988). Serum albumin acts as a necessary phospholipid carrier for both paf release (Benveniste, J. et al., 1972; Benveniste, J., 1974) and specific paf binding (Korth and Benveniste, 1987) and seems to inhibit the pafdegrading enzyme, acetylhydrolase (Wardlow et al., 1986). Different specific antagonists, such as the paf analogue CV 3988 (Terashita et al., 1983), the naturally occurring kad-surenone (Shen et al., 1985) and Ginkgolides (Nunez et al., 1986; Korth and Benveniste, 1987; Korth et al., 1988 c; Braquet and Godfroid, 1987), as well as synthetic hetrazepines and calcium-blocking agents, inhibited platelet activation and paf binding (Casals-Stenzel and Weber, 1987; Korth et al., 1987 and 1988 b; Wade et al., 1986). Binding studies with radiolabeled antagonists supported the existence of paf receptors in platelets (Hwang et al., 1986 a). A close correlation between inhibition of paf-induced platelet aggregation and binding of labelled paf suggested the importance of the putative paf receptor for platelet activation (Korth et al., 1988 c). Paf receptors were coupled with modulation of phospholipase C activity (Morrison and Shukla, 1988).


Human Platelet Human Endothelial Cell Rabbit Platelet Bovine Pulmonary Artery Endothelial Cell Intact Human Platelet 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ruth Korth
    • 1
  • M. Hirafuji
    • 3
  • J. Bidault
    • 1
  • B. Canton
    • 2
  • F. Russo-Marie
    • 2
  • J. Benveniste
    • 1
  1. 1.INSERM U 200Universite de Paris SudClamartFrance
  2. 2.INSERM U 285Unité associée Institut PasteurParisFrance
  3. 3.Dental University of TohokuSendaiJapan

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