Advertisement

Vascular Endothelial Surface Proteins in the Perfused Rabbit Lung

  • C. Norman Gillis
  • Marilyn P. Merker
  • William W. Carley

Abstract

Endothelial cells lining blood vessels interact with a wide range of blood borne substrates and cells. Underlying this dialogue is a complex mosaic of endothelial cell receptors, enzymes and membrane transport systems which serve to orchestrate crucial physiological and pathophysiological responses of the organism. Even a selected list of endothelial cell membrane receptors and transport systems (Table 1) emphasizes the diversity of potential functions they represent. For example, endothelial cells are believed to be involved in hemostasis (Nawroth et al., 1984), regulation of vascular tone (Gillis and Pitt, 1982; Furchgott, 1984), initiation of inflammation and response to immune mediators (Pober et al., 1986; Collins et al., 1986). Endothelial cell 5-HT (Crone and Levitt, 1984) and ß adrenergic receptors (Bottaro et al., 1986) have been implicated in regulation of vascular permeability. Insulin receptors apparently participate in transport of the hormone to subendothelial sites (King and Johnson, 1985), and neutrophil adhesion receptors are expressed in response to monokines, presumably as part of the inflammatory response (Pober et al., 1986).

Keywords

Angiotensin Converting Enzyme Microvascular Endothelial Cell Rabbit Lung Lectin Affinity Endothelial Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ashton, J.H., Pitt, B.R. and Gillis, C.N.. Apparent kinetics of angiotensin converting enzyme: hydrolysis of [3H] benzoyl-phenylalanyl-alanyl-proline in the isolated perfused lung. J. Pharmacol. Exp. Ther. 232:602–607, 1985.PubMedGoogle Scholar
  2. Belloni, P.N. and Nicolson, G.L.. Differential expression of cell surface glycoproteins on various organ derived microvascular endothelia and endothelial cell cultures. J. Cell Physiol. 136:398–410, 1988.PubMedCrossRefGoogle Scholar
  3. Bevan, J.A. and Duckies, S.P. Evidence for alpha adrenergic receptors on intimai endothelium. Blood Vessels. 12:307–310, 1975.PubMedGoogle Scholar
  4. Bianchi, C., Gutkowska, J., Thibault, G., Garcia, R., Genest, J. and Cantin, M. Radioautographic localization of 1251-natriuretic factor (ANF) in rat tissues. Histochemistry. 82:441–452, 1985.PubMedCrossRefGoogle Scholar
  5. Bottaro, D., Shepro, D., Peterson, S. and Hechtman, H. Serotonin, norepinephrine, and histamine mediation of endothelial cell barrier function in vitro. J. Cell. Physiol. 128:189–194, 1986.PubMedCrossRefGoogle Scholar
  6. Braquet, P., Touqui, L., Shen, T.Y. and Vargaftig, B.B. Perspectives in platelet-activating factor research. Pharmacol. Rev. 39:97–145, 1987.PubMedGoogle Scholar
  7. Carley, W., Merker, M. and Gillis, C.N. Mapping of vascular endothelial surface proteins in the intact rabbit lung. FASEB. J. 2:A1189, 1988.Google Scholar
  8. Carley, W.W., Dolci, E.D. and Palade, G.E.. Differences in polypeptide labeling between cultured endothelia derived from large and small vessels. J. Cell Biol. 105:326A, 1987.Google Scholar
  9. Catravas, J.D. and Aronstam, R.S. Muscarinic systems of the pulmonary endothelium. In: Pulmonary Endothelium in Health and Disease, edited by Ryan, U.S., Marcel Dekker, Inc, New York, 307–326, 1987.Google Scholar
  10. Collins, T., Lapierre, L., Fiers, A.W. Strominger, J.L. and Pober, J.S. Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A, B antigens in vascular endothelial cells and dermal fibroblasts in vitro. Proc. Nat. Acad. Sci. 83:446–450, 1986.PubMedCrossRefGoogle Scholar
  11. Cook, D.R., Howell, R.E. and Gillis, C.N. Xanthine oxidase-induced lung injury inhibits removal of 5-hydroxytryptamine from the pulmonary circulation. Anesth. Analg. 61:666–670, 1982.PubMedGoogle Scholar
  12. Crone, C. and Levitt, D.G. Capillary permeability to small solutes. In: Handbook of Physiology. Section 4: The Microcirculation The Cardiovascular System, edited by Fishman, A.P. and A.B. Fisher. Amer. Physiol. Soc, Bethesda, Md., 411–466, 1984.Google Scholar
  13. Folkman, J. and Klagsbrun, M. Angiogenic factors. Science. 35:442–447, 1987.CrossRefGoogle Scholar
  14. Furchgott, R.F. Role of endothelium in the responses of vascular smooth muscle to drugs. Ann. Rev. Pharmacol. 24:175–797, 1984.CrossRefGoogle Scholar
  15. Gerritson, M.E. Functional heterogeneity of vascular endothelial cells. Biochem. Pharmacol. 36:2701–2711, 1987.CrossRefGoogle Scholar
  16. Ghitescu, L., Fixman, A., Simionescu, M. and Simionescu, N. Specific binding sites for albumin restricted to plasmalemmal vesicles of continuous capillary endothelium: receptor-mediated transcytosis. J. Cell Biology. 102:1304–1311, 1986.CrossRefGoogle Scholar
  17. Gillis, C.N. Pharmacological aspects of metabolic processes in the pulmonary circulation. Ann. Rev. Pharmacol. 26:183–200, 1986.CrossRefGoogle Scholar
  18. Gillis, C.N., Havill, A.H. and Moalli, R. Effect of nafazatrom and indomethacin on pulmonary removal of prostaglandin E1 after endotoxin in rabbits. Brit. J. Pharmacol. 91:721–78, 1987.CrossRefGoogle Scholar
  19. Gillis, C.N. and Pitt, B.R. The fate of circulating amines within the pulmonary circulation. Ann. Rev. Physiol. 44:269–281, 1982.CrossRefGoogle Scholar
  20. Gumkowski, F., Kaminska, G., Kaminski, M., Morrissey, L.W., and Auerbach, R. Heterogeneity of mouse vascular endothelium. Blood Vessels. 24:11–23, 1987.PubMedGoogle Scholar
  21. Hellewell, P.G. and Pearson, J.D. Adenine nucleotides and the pulmonary endothelium. In: Pulmonary Endothelium in Health and Disease, edited by Ryan, U.S., Marcel Dekker, Inc, New York, 327–348, 1987.Google Scholar
  22. King, G.L. and Johnson, S.M. Receptor mediated transport of insulin across endothelial cells. Science. 227:1583–1586, 1985.PubMedCrossRefGoogle Scholar
  23. Kulaga, H., Sogn, J.A., Weissman, J.D., Marche, P., LeGuern, N.C., Long, E.O. and Kindt, T.J. Expression patterns of MHC class II genes in rabbit tissues indicate close homology to human counterparts. J. Immunol. 139:587–592, 1987.PubMedGoogle Scholar
  24. Lanzillo, J.J. and Fanburg, B.L. The estimation and comparison of molecular weight of angiotensin converting enzyme by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochim. Biophys. Acta. 439:125–132, 1976.PubMedCrossRefGoogle Scholar
  25. Leguern, C., Weissman, J.D., Marche, P.N., Jouvin-Marche, E., Laverriere, A., Bagnato, M.R. and T.J. Kindt. Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQ. Immunogenetics. 25:104–109, 1987.PubMedCrossRefGoogle Scholar
  26. Lobel, S.A. and Knight, K.L. The role of rabbit Ia molecules inimmune functions as determined with the use of an anti-la monoclonal antibody. Immunol. 51:35–43, 1984.Google Scholar
  27. Merker, M., Carley, W.W. and Gillis, C.N. In situ iodination of angiotensin converting enzyme and other pulmonary endothelial membrane proteins. Biochem. Pharmacol. 38:983–992, 1989.PubMedCrossRefGoogle Scholar
  28. Nawroth, P.P., Handley, D.A., Esmon C.T. and Stern, D.M. Interleukin 1 induces endothelial cell procoagulant activity while suppressing cell-surface anticoagulant activity. Proc. Nat. Acad. Sci. 83:3460–3464, 1986.PubMedCrossRefGoogle Scholar
  29. Pober, J.S., Collins, T., Gimbrone, M.A., Libby, P. and Reiss, C.S. Inducible expression of class II major histocompatability complex antigens and the immunogenicity of vascular endothelium. Transplantation. 41:141–146, 1986.PubMedCrossRefGoogle Scholar
  30. Ryan, U.S. Metabolic activities of the pulmonary endothelium: modulations of structure and function. Ann. Rev. Physiol. 48:263–277, 1986.CrossRefGoogle Scholar
  31. Simionescu, M. and Simionescu, N. Functions of the endothelial cell surface. In: Ann. Rev. Physiol., edited by Berne, R.M. Annual Reviews, Palo Alto, CA, 279–293, 1986.Google Scholar
  32. Soffer, R.L. and Das, M. Pulmonary angiotensin-converting enzyme antienzyme antibody. Biochemistry. 15:5088–5094, 1976.PubMedCrossRefGoogle Scholar
  33. Tanoue, L., Merker, M., Carley, W. and Gillis, C.N. Isolation of rabbit pulmonary microvascular endothelial cells and characterization of angiotensin converting enzyme activity. FASEB. J. 2:A298, 1988.Google Scholar
  34. Turrin, M. and Gillis, C.N. Removal of atrial natriuretic factor by perfused rabbit lungs in situ. Biochem. Biophys. Acta. 140:868–873, 1986.Google Scholar
  35. Voyta, J.C., Via, D.P., Butterfield, C.E. and Zetter, B.R. Identification and isolation of endothelial cells based on their increased uptake of acetylated low density lipoproteins. J. Cell Biol. 99:2034–2040, 1984.PubMedCrossRefGoogle Scholar
  36. Warner, A.E. and Brain, J.D.. Intravascular pulmonary macrophages: a novel cell removes particles from blood. Amer. J. Physiol. 250:R728-R732, 1986.PubMedGoogle Scholar
  37. Weibel, E.R. Lung Cell Biology. In: Handbook of Physiology. Section 3: The Respiratory System: Circulation and Non-respiratory Functions, ed. by Amer. Physiol. Soc, Bethesda, MD, 1984, 47–91.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • C. Norman Gillis
    • 1
  • Marilyn P. Merker
    • 1
  • William W. Carley
    • 1
  1. 1.Yale University School of MedicineNew HavenUSA

Personalised recommendations