Receptors for Extracellular Matrix Proteins in Endothelial Cells
The basement membrane of endothelial cells (EC) is organized as a complex network of numerous glycoproteins which include fibronectin (FN), vitronectin (VN), collagens and von Willebrand factor (vW). Beside these substrata which are directly produced by EC, fibrinogen (FG) and fibrin tend to accumulate at sites of vascular injury and may provide a new matrix over which EC can migrate and proliferate (Dejana et al., 1988). The interaction of EC with the protein components of the matrix, causes a series of cell responses as adhesion, spreading, cytoskeletal organization and cell migration. These multiple cellular reactions appear to be mediated by specific receptors which on the outer side of the membrane specifically recognize and bind different components of the extracellular matrix and, on the cytoplasmic side, link a chain of proteins involved in the mechanism of adhesion and cytoskeletal organization (Geiger, 1985).
KeywordsHuman Placenta Cytoskeletal Organization Adhesion Plaque Vitronectin Receptor Interference Reflection Microscopy
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- Conforti, G., Zanetti, A., Colella, S., Abbadini, M., Marchisio, P.C., Pytela, R., Giancotti, F., Tarone, G., Languino, L.R. and Dejana, E. Interaction of fibronectin with cultured human endothelial cells. Characterization of the specific receptor. Blood in press, 1989.Google Scholar
- Dejana, E., Languino, L.R., Polentarutti, G., Balconi, G., Ryckwaert, J.J., Larrieu, M.J., Donati, M.B., Mantovani, A. and Marguerie, G. Interaction between fibrinogen and cultured endothelial cells. Induction of migration and specific binding. J. Clin. Invest. 75:11–18, 1985.PubMedCrossRefGoogle Scholar
- Dejana, E., Zanetti, A., Conforti, G. Biochemical and functional characteristics of fibrinogen interaction with endothelial cells. Haemostasis. 1988. In press.Google Scholar
- Languino, L.R., Colella, S., Zanetti, A., Andrieux, A., Ryckewaert, J.J., Charon, J.J., Marchisio, P.C., Plow, E.F., Ginsberg, M.H., Marguerie, G., and Dejana, E. Fibrinogen-endothelial cell interaction in vitro: a pathway mediated by Arg-Gly-Asp recognition specificity. Blood. In press. 1988.Google Scholar
- Preissner, K.T., Anders, E. and Muller-Berghaus G. Interaction of S protein/vitronectin with cultured endothelial cells: promotion of attachment and specific binding. Thromb. Haemost. 58 (1): 227, 1987.Google Scholar