The Role of Basement Membrane in Angiogenesis

  • Michael E. Maragoudakis


Many laboratories have contributed to the present day understanding of the individual steps involved in angiogenesis (D’Amore & Thomson, 1986). These sequential steps can be summarized as follows:
  1. 1.

    Local degradation of basement membranes of the parent vessel, usually a venule, by specific enzymes released by the “activated” endothelial cells under the influence of an angiogenic stimulus.

  2. 2.

    Increase in the number of organelles and pseudopodes of endothelial cells that protrude through the “holes” of basement membrane of the parent vessel.

  3. 3.

    Migration of endothelial cells from the vessel wall into the interstitial space toward the angiogenic stimulus.

  4. 4.

    Alignment of endothelial cells to form a sprout, which elongates. The migrating endothelial cells are at the tip and behind are the endothelial cells in mitosis.

  5. 5.

    A lumen is formed by each endothelial cell in the sprout and the individual sprouts join with each other to form a loop through which blood begins to flow.

  6. 6.

    Pericytes migrate along the capillary sprout and become associated with the abluminal surface of the endothelial cells.

  7. 7.

    The final step is the formation of basement membranes by the endothelial cells and probably the pericytes.



Basement Membrane Capillary Endothelial Cell Vascular Density Parent Vessel Extracellular Matrice 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Michael E. Maragoudakis
    • 1
  1. 1.Department of PharmacologyUniversity of Patras Medical SchoolPatrasGreece

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