Lipid Structures and Lipid-Protein Interactions in Thylakoid Membranes

  • Kleoniki Gounaris


Biological membranes are essentially lipoprotein structures and, in general, are described in terms of the fluid mosaic model. In this current view of biomembrane structure, the lipid component forms a closed, stable bilayer while in a fluid liquid-crystalline condition. It thereby provides a regulated and controlled internal environment as well as a matrix for the lateral diffusion of membrane proteins. Such a model implies that any enzyme residing in the bilayer must have lipid associated with the protein surface. Most biomembranes contain a large number of integral proteins and clearly the fraction of lipid involved at protein/lipid interfaces will be higher as the protein to lipid ratio of the membrane as a whole increases. Lipid-protein associations occur in all membrane systems and at the molecular level any interactions, whether hydrophobic, steric or electrostatic, must be governed by common principles.


Thylakoid Membrane Lipid Mixture Total Polar Lipid Soybean Phospholipid Hexagonal Type 
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  1. 1.
    Warren, G.B., Houslay, M.D., Metcalfe,_J.C. and Birdsall, N.J.M. (1975) Cholesterol is excluded from the phospholipid annulus surrounding an active calcium transport protein. Nature 255, 684–687Google Scholar
  2. 2.
    Cronan, J.E. and Gelmann, E.P. (1975) Physical properties of membranelipids: Biological relevance and regulation. Bacteriol. Rev. 39, 232–256Google Scholar
  3. 3.
    Taraschi, T.F., de Kruijff, B., Verkleij,A.J. and van Echteld, C.J.A. (1982) Effect of glycophorin on lipid polymorphism. A 31P NMR study. Biochim. Biophys. Acta 685, 153–161CrossRefGoogle Scholar
  4. 4.
    Israelachvili, J.N., Marcelja, S. and Horn, R.G. (1980) Physical principles of membrane organisation, Q. Rev. Biophys. 13, 121–200CrossRefGoogle Scholar
  5. 5.
    Luzzati, V. and Husson, F. (1962) The structure of the liquid-crystalline phases of lipid-water systems. J. Cell Biol. 12, 207–219CrossRefGoogle Scholar
  6. 6.
    Cullis. P.R. and de Kruíjff, B. (1978) The polymorphic phase behaviour of phosphatidylethanolamines of natural and synthetic origin. A31PNMR study Biochim. Biophys. Acta 513, 31–42CrossRefGoogle Scholar
  7. 7.
    Cullis, P.R., Verkleij, A.J. and Vernergaert, P.H.J.Th. (1978) Polymorphic phase behaviour of cardiolipin as detected by 31P-NMR and freeze-fracture techniques. Effects of calcium, dibucaine and chlorpromazine. Biochim. Biophys. Acta 513, 11–20CrossRefGoogle Scholar
  8. 8.
    Wieslander, A. Ulmins, J., Lindblom, G. and Fontel, K. (1978) Water binding and phase structures for different Acholeplasma Laidlawii membrane lipids studied by deuteron nuclear magnetic resonance and X-ray diffraction. Biochim. Biophys. Acta 512, 241–253Google Scholar
  9. 9.
    Deamer, D.W., Leonard, R., Tardieu, A. and Branton, D. (1970) Lamellar and hexagonal lipid phases visualised by freeze-etching. Biochim. Biophys. Acta 219, 47–60CrossRefGoogle Scholar
  10. 10.
    Papahadjopoulos, D., Vail, W.J., Pangborn, W.A. and Poste, G. (1976) Studies on membrane fusion. Induction of fusion in pure phospholipid membranes by calcium ions and other divalent metals. Biochim. Biophys. Acta 448, 265–283CrossRefGoogle Scholar
  11. 11.
    Vail, W.J. and Stollery, J.G. (1979) Phase changes of cardiolipin vesicles mediated by divalent cations. Biochim. Biophys. Acta 551, 74–78CrossRefGoogle Scholar
  12. 12.
    Sen, A., Williams, W.P., Brain, A.P.R., Dickens, M.J. and Quinn, P.J. (1981) Formation of inverted micelles in dispersions of mixed galactolipids. Nature 293, 488–490CrossRefGoogle Scholar
  13. 13.
    Verkleij, A.J., Mombers, C., Leunissen-Bijrelt, L. and Ververgaert, P.J.J.Th. (1979) Lipidic intramembranous particles. Nature, 279, 162–163CrossRefGoogle Scholar
  14. 14.
    Sen, A., Brain, A.P.R., Quinn, P.J. and Williams, W.P. (1982) Formation of inverted lipid micelles in aqueous dispersions of mixed sn-3-galactosyldiacylglycerols induced by heat and ethylene glycol. Biochim. Biophys. Acta 686, 215–224CrossRefGoogle Scholar
  15. 15.
    Barber, J. (1980) Membrane surface charges and potentials in relation to photosynthesis. Biochim. Biophys. Acta 594, 253–308Google Scholar
  16. 16.
    Gounaris, K., Sen, A., Brain, A.P.R., Quinn, P.J. and Williams, W.P. (1983) The formation of non-bilayer structures in total polar lipid extracts of chloroplast membranes. Biochim. Biophys. Acta 728, 129–139CrossRefGoogle Scholar
  17. 17.
    Gounaris, K., Mannock, D.A., Sen, A., Brain, A.P.R., Williams, W.P. and Quinn, P.J. (1983) Polyunsaturated fatty acyl residues of galactolipids are involved in the control of bilayer/non-bilayer lipid transitions in higher plant chloroplasts. Biochim. Biophys. 732, 229–242CrossRefGoogle Scholar
  18. 18.
    Gounaris, K., Brain, A.P.R., Quinn, P.J. and Williams, W.P. (1984) Structural reorganisation of chloroplast thylakoid membranes in response to heat-stress. Biochim. Biophys. Acta 766, 198–208CrossRefGoogle Scholar
  19. 19.
    Crow, L.M. and Crowe, J.H. (1982) Hydration-dependent hexagonal phase lipid in a biological membrane. Arch. Biochem. Biophys. 217, 582–587CrossRefGoogle Scholar
  20. 20.
    van Venetie, R. and Verkleij, A.J. (1982) Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study. Biochim. Biophys. Acta 692, 397–405CrossRefGoogle Scholar
  21. 21.
    Winget, G.D., Kanner, N. and Racker, E. (1977) Formation of ATP by the adenosine triphosphatase complex from spinach chloroplasts reconstituted together with bacteriorhodopsin. Biochim. Biophys. Acta 460, 490–499CrossRefGoogle Scholar
  22. 22.
    Pick, U., Gounaris, K., Admon, A. and Barber, J. (1984) Activation of the CF0–CF1 ATP synthease from spinach chloroplasts by chloroplast lipids. Biochim. Biophys. Acta 765, 12–20CrossRefGoogle Scholar
  23. 23.
    Pick, U., Gounaris, K., Weiss, M. and Barber, J. (1985) Tightly bound sulpholipids in chloroplast CF0–CF1. Biochim. Biophys. Acta 808, 415–420CrossRefGoogle Scholar
  24. 24.
    Robinson, N.C. (1982) Specificity and binding affinity of phospholipids to the high-affinity cardiolipin sites of beef heart cytochrome c oxidase. Biochemistry. 21, 184–188CrossRefGoogle Scholar
  25. 25.
    Kagawa, Y., Kandrach, A. and Racker, E. (1973) Partial resolution of the enzymes catalyzing oxidative phosphorylation. Specificity of phospholipids required for energy transfer reactions. J. Biol. Chem. 248, 676–684Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Kleoniki Gounaris
    • 1
  1. 1.AFRC Photosynthesis Research Group Department of Pure and Applied BiologyImperial College of Science and TechnologyLondonUK

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