Proton Transport-Coupled ATP Synthesis Catalyzed by the Chloroplast ATPase

  • Peter Gräber
  • Ulrike Junesch
  • Günter Schmidt
  • Petra Fromme


The membrane-bound ATPase in chloroplasts can catalyze ATP synthesis/hydrolysis coupled with a transmembrane proton transport. The detailed mechanism by which proton transport gives rise to the formation of a phosphate-anhydrid bond is still not known. We have investigated the kinetics of ATP synthesis with chloroplasts and with CFo F1 reconstituted into liposomes. From these results we want to draw conclusions about the mechanism of the reaction. The rate of ATP synthesis was measured with a rapid double-mixing system using artificially impressed transmembrane pH difference, ∆ pH, electric potential difference, ∆Ψ and phosphate potential, ∆Gp (1).


Phosphate Potential Chemiosmotic Theory Electrochemical Potential Difference Chloroplast ATPase Centrifugation Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    P. Gräber, U. Junesch and G.H. Schatz, Ber. Bunsenges. Phys. Chem. 88: 599 (1984)Google Scholar
  2. 2.
    S.R. Ketcham, J.W. Davenport, K. Warncke and R.E. McCarty, J. Biol. Chem. 259: 7286 (1984)Google Scholar
  3. 3.
    C.M. Nalin and R.E. McCarty, J. Biol. Chem. 259: 7275 (1984)Google Scholar
  4. 4.
    J.V. Moroney, C.S. Fullmer and R.E. McCarty, J. Biol. Chem. 259: 7281 (1984)Google Scholar
  5. 5.
    J.L. Arna and R.H. Vallejos, J. Biol. Chem. 257: 1125 (1982)Google Scholar
  6. 6.
    U. Petrack and F. Lipmann in: Light and Life, McElroy, V.D. and Glass, H.B., eds., pp. 621–630, Hopkins, Baltimore, MD (1961)Google Scholar
  7. 7.
    L. Packer and R.M. Merchant, J. Biol. Chem. 239: 2061 (1963)Google Scholar
  8. 8.
    C. Carmeli, FEBS Lett. 7: 297 T1970Google Scholar
  9. 9.
    T. Bakker-Grunwald and K. van Dam, Biochim. Biophys. Acta 292: 808 (1973)CrossRefGoogle Scholar
  10. 10.
    J.W. Davenport and R.E. McCarty, J. Biol. Chem. 256: 8947 (1981)Google Scholar
  11. 11.
    U. Junesch and P. Gräber, FEBS Lett. in press (1985)Google Scholar
  12. 12.
    U. Junesch and P. Gräber, Biochim. Biophys. Acta, in press (1985)Google Scholar
  13. 13.
    P. Gräber, E. Schlodder and H.T. Witt, Biochim. Biophys. Acta 461: 426 (1977)CrossRefGoogle Scholar
  14. 14.
    B. Rumberg and U. Becher in: H+-ATPase/synthase; structure, function, regulation, S. Papa et al., eds.), pp. 421–430, Adriatica Editrice, Bari, Italy (1984)Google Scholar
  15. 15.
    C. Carmeli and Y. Lifshitz, Biochim. Biophys. Acta 267: 86 (1972)CrossRefGoogle Scholar
  16. 16.
    S. Bickel-Sandkötter and H. Strotmann, FEBS Lett. 126: 188 (1981)CrossRefGoogle Scholar
  17. 17.
    J. Schumann and H. Strotmann in: Photosynthesis, Akoyunoglou, G., ed., Vol. II, pp. 881–892, Balaban Intern. Sci. Serv., Philadelpha, PA (1981)Google Scholar
  18. 18.
    N. Shavit, C. Aflalo and D. Bar-Zvi in: Energy Coupling in Photosynthesis, Selman, B.R. and Selman-Reimer, S., eds., pp.197–207Google Scholar
  19. 19.
    K.R. Dunham and B.R. Selman, J. Biol. Chem. 256: 212 (1981)Google Scholar
  20. 20.
    P. Gräber and E. Schlodder in: Photosynthesis, Akoyunoglou, G., ed., Vol. II, pp. 867–880, Balaban Intern. Sci. Serv., Philadelphia, PA (1981)Google Scholar
  21. 21.
    P. Mitchell, Nature 191: 144 (1961)CrossRefGoogle Scholar
  22. 22.
    G.D. Winget, N. Kanner and E. Racker, Biochim. Biophys. Acta 460: 490 (1977)CrossRefGoogle Scholar
  23. 23.
    T.G. Dewey and G.G. Hammes, J. Biol. Chem. 256: 8941 (1981)Google Scholar
  24. 24.
    G. Hauska, D. Samoray, G. Orlich and N. Nelson, Eur. J. Biochem. 111: 535 (1980)CrossRefGoogle Scholar
  25. 25.
    G. Schmidt and P. Gräber, Biochim.Biophys. Acta 808: 46 (1985)CrossRefGoogle Scholar
  26. 26.
    G. Grubmeyer, R.L. Cross and M.S. Penefsky, J. Biol. Chem. 257: 12092 (1982)Google Scholar
  27. 27.
    P. Gräber and P. Fromme, FEBS Lett. in press (1985)Google Scholar
  28. 28.
    P. Boyer in: Bioenergetics of Membranes, C.P. Lee, ed., Addison-Wesley (1979)Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Peter Gräber
    • 1
  • Ulrike Junesch
    • 1
  • Günter Schmidt
    • 1
  • Petra Fromme
    • 1
  1. 1.Max-Volmer-Institut für Biophysikalische und Physikalische ChemieTechnische Universität BerlinBerlin 12Germany

Personalised recommendations