Control of Mitochondrial Respiration by the Protonmotive Force

  • K. van Dam
  • H. Woelders
  • A. M. A. F. Colen


Since the development of the chemiosmotic hypothesis by Mitchell (1) a central role in energy conservation has been attributed to protons (2). The experiments, designed to verify this role, have been very successful in demonstrating that movement of protons generally accompanies the process of energy transduction in mitochondria, chloroplasts and bacteria. However, up to this moment, there are a number of observations that are difficult to reconcile with the original scheme proposed by Mitchell. A discussion of these anomalies can be found in a review by Westerhoff et al. (3).


Respiratory Chain Original Scheme Energy Transduction Bulk Aqueous Phase Experimental Argument 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Mitchell, P., Nature (London) 191, 144–148 (1961).CrossRefGoogle Scholar
  2. 2.
    Boyer, P.D., B.Chance, L.Ernster, E.Racker and E.C.Slater, Ann.Rev. Biochem. 46, 955–1026 (1977).CrossRefGoogle Scholar
  3. 3.
    Westerhoff, H.V., B.A.Melandri, G.Venturoli, G.F.Azzone and D.B.Kell, Biochim.Biophys.Acta 768, 257–292 (1984).Google Scholar
  4. 4.
    Van Dam, K. and H.V.Westerhoff, in: Bioenergetics, L.Ernster, ed., Elsevier, Amsterdam, p. 1–27 (1984).Google Scholar
  5. 5.
    Williams, R.J.P., Biochim.Biophys.Acta 505, 1–44 (1978).Google Scholar
  6. 6.
    Rottenberg, H., in: Frontiers in biological energetics, Vol.1, P.L.Dutton, J.S.Leigh and S.Scarpa, eds., Academic Press, New York, pp.403–412 (1978).Google Scholar
  7. 7.
    Kell, D.B. and Morris, J.G., in:Vectorial reactions in electron and ion transport in mitochondria and bacteria, F.Palmieri, E.Quagliariello, N.Siliprandi and E.C.Slater, eds., Elsevier, Amsterdam, pp.339–347 (1981).Google Scholar
  8. 8.
    Van Dam, K., H.Woelders, A.M.A.F.Colen and H.V.Westerhoff, Biochem. Soc.Trans. 12, 401–402 (1984).Google Scholar
  9. 9.
    Berden, J.A., M.A.Herweijer and J.B.J.W.Cornelissen, in: H+-ATPase, S.Papa, K.Altendorf, L.Ernster and L.Packer, eds., Adriatica Editrice, Bari, pp. 339–348 (1984).Google Scholar
  10. 10.
    Woelders, H., W.J.van der Zande, A.M.A.F.Colen, R.J.A.Wanders and K.van Dam, FEBS Letters 179, 278–282 (1985).CrossRefGoogle Scholar
  11. 11.
    Padan, E. and H.Rottenberg, Eur.J.Biochem. 40, 431–437 (1973).CrossRefGoogle Scholar
  12. 12.
    Azzone, G.F., T.Pozzan, S.Massari and M.Bragadin, Biochim.Biophys. Acta 501, 296–306 (1978).CrossRefGoogle Scholar
  13. 13.
    Van Dam, K., H.V.Westerhoff, K.Krab, R.van der Meer and J.C.Arents, Biochim.Biophys.Acta 591, 240–250.Google Scholar
  14. 14.
    Tsou, C.S., Mitochondrial membrane processes, Ph.D.Thesis, University of Amsterdam, Mondeel Offset Drukkerij, Amsterdam (1970).Google Scholar
  15. 15.
    Baum, H., H.S.Hall, J.Nalder and R.B.Beechey, in: Energy transduction in respiration and photosynthesis,E.Quagliariello, S.Papa and G.S.Rossi, eds., Adriatica Editrice, Bari, pp. 747–755 (1971).Google Scholar
  16. 16.
    Hitchens, G.D. and D.B.Kell, Biochem.J. 206, 351–357 (1982).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • K. van Dam
    • 1
  • H. Woelders
    • 1
  • A. M. A. F. Colen
    • 1
  1. 1.Laboratory of BiochemistryUniversity of AmsterdamAmsterdamThe Netherlands

Personalised recommendations