Regulation of Protease Nexin-1 Activity and Target Protease Specificity by the Extracellular Matrix

  • Dennis D. Cunningham
  • David H. Farrell
  • Steven L. Wagner
Part of the NATO ASI Series book series (NSSA, volume 191)


PN-1 is a 44 kDa protein protease inhibitor that is synthesized and secreted by a variety of cultured cells including fibroblasts, smooth muscle cells and astrocytes. 1–4 It rapidly inhibits thrombin, urokinase and plasmin by forming SDS-stable complexes with the catalytic site serine of the protease.1–5 The protease-PN-1 complexes bind back to the cells, via the PN-1 moiety of the complex, and are rapidly internalized and degraded.2 This provides a mechanism to regulate and clear certain serine proteases in the extracellular environment.


Neuroblastoma Cell Heparan Sulfate Neurite Outgrowth Human Fibroblast Target Protease 


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Dennis D. Cunningham
    • 1
  • David H. Farrell
    • 1
  • Steven L. Wagner
    • 1
  1. 1.Department of Microbiology and Molecular Genetics College of MedicineUniversity of CaliforniaIrvineUSA

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