Protease Nexin I Immunostaining in Alzheimer’s Disease
The NATO Conference which provided the impetus for this book was focused on protease and protease inhibitor activity in the nervous system. The conference covered both the Peripheral Nervous System (PNS) and the Central Nervous System (CNS) and addressed both developmental and pathological conditions. A recurrent theme throughout the conference was that of a crucial balance between proteolytic enzyme and inhibitor activity. Another theme of the conference was that of neuron-glia interaction. This chapter will report on immunohistochemical studies on Protease Nexin I (PN I), a glial-derived serine protease inhibitor with neurite promoting activity, in Alzheimer brain and will discuss the results in light of material presented at the conference.
KeywordsSucrose EDTA Dementia Heparin Serine
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- 3.V.W. Henderson, and C.E. Finch, The neurobiology of Alzheimer’s disease. J Neurosurg 70: 335353 (1989).Google Scholar
- 16.D. Monard, F. Solomon, M. Rentsch, and R. Gysin, Glia-induced morphological differentiation in neuroblastoma cells. Proc Nat Acad Sci USA 70: 18941897 (1973).Google Scholar
- 20.L.P. Mulligan, D.E. Rosenblatt, and D. Johnson, Protease nexin I-like activity in cultured Schwann cells. SubmittedGoogle Scholar
- 25.LA. Sternberger, P.H. Hardy Jr, J.J. Cuculis, and H.G. Meyer, The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem Cytochem 18: 315–346 (1970).CrossRefPubMedGoogle Scholar
- 28.A.D. Snow, MAR. Henderson, and D Nochlin et al. The presence of heparan sulfate proteoglycan in the neuritic plaques and congophilic angiopathy in Alzheimer’s disease. Am. J. Path. 133: 456462 (1988).Google Scholar
- 30.S.L. Wagner, A.L. Lau, and D.D. Cunningham, Binding of protease nexin-1 to the fibroblast surface alters its target proteinase specificity. J Biol Chem: 2 64: 611–615 (1989).Google Scholar