Evidence for the Molecular Dissociation of Binding and Post-Binding Functions in Cytotoxic T Lymphocytes

  • John H. Russell
  • Rawleigh C. Howe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 187)


The interaction between the cytotoxic T lymphocyte (CTL) and its target cell has been a model of immune cellular interaction for several years because of the relatively short time course between the actual receptor-ligand interaction and its functional expression in target cell lysis. The nature of the lethal event within the target remains unclear but recent experiments from our laboratory and others have suggested that CTL mediated lysis involves not only the production of an osmotic lesion in the plasma membrane but also rapid changes in nuclear and chromatin structure (see ref. #1 for review). This nuclear lesion distinguishes CTL mediated lysis from cell death mediated by Ab+C1 or hypotonic shock.


Lytic Activity Mixed Lymphocyte Culture Lytic Process Lytic State Weekly Stimulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Russell, J.H. 1983. Internal disintegration model of cytotoxic lymphocyte-induced target damage. Immunol. Rev. 72:97.Google Scholar
  2. 2.
    Golstein, P. and E.T. Smith. 1977. Mechanisms of T-cell mediated cytolysis: the lethal hit stage. Contemp. Top. Immunol. 4:273.Google Scholar
  3. 3.
    Martz, E. 1977. Mechanism of specific tumor cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp. Top. Immunol. 4:301.Google Scholar
  4. 4.
    Russell, J.H. and C.B. Dobos. 1980. Mechanisms of immune lysis. II. CTL-induced nuclear disintegration of the target begins within minutes of cell contact. J. Immunol. 125:1256.Google Scholar
  5. 5.
    Reinherz, E.L., S. C., Meuer and S.F. Schlossman. 1983. The human T cell receptor: analysis with cytotoxic T cell clones. Immunol. Rev. 74:83.Google Scholar
  6. 6.
    Martz, E., W. Heagy and S.H. Gromkowski. 1983. The mechanism of CTL-mediated killing: monoclonal antibody analysis of the roles of killer and target-cell membrane proteins. Immunol. Rev. 72:73.Google Scholar
  7. 7.
    Tsoukas, C.D., P.A. Carson, S. Fong and J.H. Vaughan. 1982. Molecular interactions in human T cell-mediated cytotoxicity to EBV. II. Monoclonal antibody 0KT3 inhibits a post-killer-target recognition/adhesion step. J. Immunol. 129:1421.PubMedGoogle Scholar
  8. 8.
    Harris, D.T., H.R., MacDonald and J.-C., Cerottin. 1984. Direct transfer of antigen specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones. J. Exp. Med. 159:261.Google Scholar
  9. 9.
    Dennert, G. and E. Podack. 1983. Cytolysis by H-2 specific T killer cells: Assembly of tubular complexes or target membranes. J. Exp. Med. 157:1483.Google Scholar
  10. 10.
    Glasebrook, A.L., M. Sarmiento, M.R. Loken, et al., 1980. Murine T lymphocyte clones with distinct immunological functions. Immunol. Rev. 51:93.Google Scholar
  11. 11.
    Howe, R.C. and J.H. Russell. 1983. Isolation of alloreactive CTL clones with cyclical changes in lytic activity. J. Immunol. 131:2141.PubMedGoogle Scholar
  12. 12.
    Russell, J.H. and C.B. Dobos. 1983. Characterization of a “heteroclitic” cytotoxic lymphocyte clone: heterogeneity of receptors or signals? J. Immunol. 130:538.PubMedGoogle Scholar
  13. 13.
    Bonavida, B., T.P. Bradley and E.A. Grimon. 1983. Frequency determination of killer cells by a single-cell cytotoxic assay. Meth. in Enzymology. 93:270.Google Scholar
  14. 14.
    Russell, J.H. 1984. Phorbol esters inactivate the lytic apparatus of cytotoxic T lymphocytes. J. Immunol, (in press).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • John H. Russell
    • 1
  • Rawleigh C. Howe
    • 1
  1. 1.Department of PharmacologyWashington University Medical SchoolSt. LouisUSA

Personalised recommendations