p215 and p24: Two Membrane-Associated Proteins Expressed on Cloned Cytolytic T-Cells but not on Cloned Helper T-Cells

  • Maurice K. Gately
  • Michael D. Dick
  • Tomiya Masuno
  • Richard M. McCarron
  • Beatrice Macchi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 187)


It has been shown that the mechanism by which cytolytic T lymphocytes (CTL)2 lyse target cells can be divided into three successive steps (reviewed in 1): (i) binding of CTL to target cells (adhesion formation), (ii) the action of CTL upon target cells to cause the targets to become irreversibly committed to lyse (programming for lysis, also called the lethal hit), and (iii) actual target cell lysis which occurs independent of continued contact between target cells and CTL. However, the molecular mechanisms by which these processes occur are poorly understood. Studies with monoclonal antibodies which can inhibit lysis in the absence of complement have permitted identification of several molecules which appear to play a role in the cytolytic mechanism (reviewed in 2–6). These include Lyt 2 and its human homologues (2–5), LFA-1 (2–5), T3 (7, 8), and clonotypic molecules which likely represent the T cell receptor (9, 10). Lyt 2 and LFA-1 appear to play a role in the binding of CTL to target cells (3, 5) whereas T3 may serve as a triggering link between the antigen-specific receptor and the lethal hit (6). No molecules have yet been identified which have been shown unambiguously to be involved in the actual delivery of the lethal hit.


Membrane Fraction Apparent Molecular Weight Electrophoretic Profile Immunoprecipitation Study Natural Killer Cell Lysis 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Maurice K. Gately
    • 1
  • Michael D. Dick
    • 1
  • Tomiya Masuno
    • 1
  • Richard M. McCarron
    • 1
  • Beatrice Macchi
    • 1
  1. 1.Surgical Neurology Branch, National Institute of Neurological and Communicative Disorders and StrokeNational Institutes of HealthBethesdaUSA

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