The Plasma Membrane ‘Skeleton’ of Tumor and Lymphoid Cells: A Role in Cell Lysis?

  • Matthew F. Mescher
  • John R. Apgar
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 187)


A detailed understanding of the structure and dynamics of the surface membrane of cells is crucial to study of the mechanisms of cell mediated cytotoxicity. The dynamics of the membrane receptors, target cell antigens and accessory proteins almost certainly affect the efficiency of establishing and maintaining cell-cell contact and delivery of the transmembrane signal resulting in triggering of the lytic mechanisms. Furthermore, whether the primary event involved in target cell lysis is formation of membrane lesions (pore formation) or transfer of a lytic component (enzyme?) from the effector cell to the target, the molecular requirements for these events will certainly depend upon the structure and dynamics of the target cell plasma membrane.


Lymphoid Cell Insoluble Fraction Membrane Matrix Peripheral Layer Membrane Skeleton 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    S.J. Singer and G.L. Nicolson, The fluid mosaic model of the structure of cell membranes, Science. 175:720 (1972).PubMedCrossRefGoogle Scholar
  2. 2.
    M.S. Bretscher and M.C. Raff, Mammalian plasma membranes, Nature. 258:43 (1975).PubMedCrossRefGoogle Scholar
  3. 3.
    S.E. Lux, Dissecting the red cell membrane skeleton, Nature. 281:426 (1979).PubMedCrossRefGoogle Scholar
  4. 4.
    D. Branton, C.M. Cohen and J. Tyler, Interaction of cyto- skeletal proteins on the human erythrocyte membrane, Cell. 24:24 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    Y. Lange, R.A. Hadesman and T.L. Steck, Role of the reticulum in the stability and shape of the isolated human erythrocyte membrane, J. Cell Biol. 92:714 (1982).PubMedCrossRefGoogle Scholar
  6. 6.
    D. Shotton, B. Burke and D. Branton, Molecular structure of spectrin, J. Mol. Biol. 131:303 (1979).PubMedCrossRefGoogle Scholar
  7. 7.
    P. Williamson, J. Bateman, K. Kozarsky, K. Mattocks, N. Hermanowicz, H.R. Choe and R.A. Shlegel, Involvement of spectrin in the maintenance of phase-state asymmetry in the erythrocyte membrane, Cell. 30:725 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    M.F. Mescher, M.J.L. Jose and S.P. Balk, Actin-containing matrix associated with the plasma membrane of murine tumor and lymphoid cells, Nature. 289:139 (1981).PubMedCrossRefGoogle Scholar
  9. 9.
    S.H. Herrmann, J.R. Apgar, J.M. Robinson and M.F. Mescher, Plasma membrane skeleton of murine tumor cells. I. Preparation and morphology of Triton shells, submitted for publication.Google Scholar
  10. 10.
    M.F. Mescher, J.R. Apgar and J.M. Robinson, Plasma membrane skeleton of murine tumor cells. II. Composition of isolated membrane skeleton and Triton shells, submitted for publication.Google Scholar
  11. 11.
    F. Lemonnier, M. Mescher, L. Sherman and S. Burakoff, The induction of cytolytic T lymphocytes with purified plasma membranes, J. Immunol. 120:1114 (1978).PubMedGoogle Scholar
  12. 12.
    M.J. Crumpton and D. Snary, Preparation and properties of lymphocyte plasma membrane, Contemp. Top. Mol. Immunol. 3:27 (1974).PubMedCrossRefGoogle Scholar
  13. 13.
    B.H. Barber and M.J. Crumpton, Actin associated with purified lymphocyte plasma membrane, FEBS Letters. 66:215 (1976).PubMedCrossRefGoogle Scholar
  14. 14.
    A.A. Davies, N.M. Wigglesworth, D. Allan, R.J. Owens and M.J. Crumpton, Nonidet P-40 extraction of lymphocyte plasma membrane, Biochem. J. 219:301 (1984).PubMedGoogle Scholar
  15. 15.
    J.R. Apgar and M.F. Mescher, Plasma membrane matrix of murine tumor cells, Fed. Proc. 43:2016 (1984).Google Scholar
  16. 16.
    A. Ben-Zeev, A. Duerr, F. Solomon and S. Penman, The outer boundry A. Ben-Zeev, A. Duerr, F. Solomon and S. Penman, The outer boundry of the cytoskeleton: A lamina derived from plasma membrane proteins, Cell. 17:859 (1979).Google Scholar
  17. 17.
    A.B. Fulton, J. Prives, S.R. Farmer and S. Penman, Developmental reorganization of the skeletal framework and its surface lamina in fusing muscle cells, J. Cell. Biol. 91:103 (1981).PubMedCrossRefGoogle Scholar
  18. 18.
    C.H. Streuli, B. Patel and D.R. Critchley, The cholera toxin receptor ganglioside GM 1 remains associated with Triton X-100 cytoskeletons of BALB/c-3T3 cells, Exp. Cell. Res. 136:247 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    V-P. Lehto, T. Vartio, R.A. Badley and I. Virtanen, Characterization of detergent-resistant surface lamina in cultured human fibroblasts. Exp. Cell Res. 143:287 (1983).PubMedCrossRefGoogle Scholar
  20. 20.
    R.J. Owens, C.J. Gallagher and M.J. Crumpton, Cellular distribution of P68, a new calcium-binding protein from lymphocytes. EMBO J. 3: n press.Google Scholar
  21. 21.
    V. Bennett, Immunoreactive forms of human erythrocyte ankyrin are present in diverse cells and tissues, Nature. 281:597 (1979).PubMedCrossRefGoogle Scholar
  22. 22.
    B. Geiger, Proteins related to the red cell cytoskeleton in non-erythroid cells, Trends Biochem. Sci. 7:388 (1982).CrossRefGoogle Scholar
  23. 23.
    E. Lazarides and W.J. Nelson, Expression of spectrin in nonerythroid cells, Cell. 31:505 (1982).PubMedCrossRefGoogle Scholar
  24. 24.
    C.L. Koski, L.E. Ramm, C.H. Hammer, M.M. Mayer and M.L. Shin, Cytolysis of nucleated cells by complement: Cell death displays multi-hit characteristics, Proc. Natl. Acad. Sci. USA. 80:3816 (1983).PubMedCrossRefGoogle Scholar
  25. 25.
    S.H. Ohanian and S.I. Schlager, Humoral immune killing of nucleated cells: Mechanisms of complement-mediated attack and target cell defense, CRC Crit. Rev. Immunol. 1:165 (1981).Google Scholar
  26. 26.
    S.H. Herrmann and M.F. Mescher, Secondary cytolytic T lymphocyte stimulation by purified H-2Kk in liposomes, Proc. Natl. Acad. Sci. USA. 78:2488 (1981).PubMedCrossRefGoogle Scholar
  27. 27.
    S.H. Herrmann and M.F. Mescher, Lymphocyte recognition of H-2 antigen in liposomes, J. Supramol. Struct, and Cell. Biochem. 16:121 (1981).CrossRefGoogle Scholar
  28. 28.
    C.J. Sanderson, Morphological aspects of lymphocyte mediated cytotoxicity, in: “Mechanisms of Cell Mediated Cytotoxicity,” W.R. Clark and P. Golstein, eds., Plenum Press, New York (1982).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Matthew F. Mescher
    • 1
  • John R. Apgar
    • 1
  1. 1.Department of PathologyHarvard Medical SchoolBostonUSA

Personalised recommendations