Functional Relationships of LYT-2 and LYT-3 Expression and T-Cell Cytotoxicity: A New Model System

  • Michael A. PalladinoJr.
  • Gerald E. Ranges
  • Herbert F. Oettgen
  • Lloyd J. Old
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 187)


Three systems of surface antigens, Lyt-1, Lyt-2 and Lyt-3, characterize cells of thymic origin in the mouse.1 Lyt-1 antigens, coded by a locus with two alleles on chromosome 19, appear to be expressed on all classes of T-cells.2 Lyt-2 and Lyt-3 antigens on the other hand, clearly distinguish subclasses of T-cells.3,4 Cytotoxic and suppressor T-cells express Lyt-2 and Lyt-3, whereas helper T-cells generally lack these antigens. Lyt-2 and Lyt-3 are coded for by a single gene or closely linked genes on chromosome 6.5


Killing Activity Mixed Lymphocyte Culture Allelic Exclusion Light Chain Variable Region Immunoglobulin Kappa Light Chain 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    E. A. Boyse, M. Miyazawa, T. Aoki, and L. J. Old, Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc. R Soc. Lond. (Biol), 170:175 (1968).CrossRefGoogle Scholar
  2. 2.
    .K. Itakura, J. J. Hutton, E. A. Boyse, and L. J. Old, Linkage groups of the theta and Ly-A loci, Nature 230:126 (1971).CrossRefGoogle Scholar
  3. 3.
    H. Cantor and E. A. Boyse, Lymphocytes as models for the study of mammalian cellular differentiation, Immunol. Rev. 33:105 (1977).PubMedCrossRefGoogle Scholar
  4. 4.
    H. Shiku, P. Kisielow, M. A. Bean, T. Takahashi, E. A. Boyse, H. F. Oettgen, and L. J. Old, Expression of T-cell differ- entation antigens on effector cells in cell mediated cytotoxicity in vitro, J. Exp. Med. 141:227 (1975).Google Scholar
  5. 5.
    K. Itakura, J. J. Hutton, E. A. Boyse, and L. J. Old, Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse, Transplantation 13:239 (1972).PubMedCrossRefGoogle Scholar
  6. 6.
    H. R. MacDonald, A. L. Glasebrook, and J. C. Cerottini, Clonal heterogeneity in the functional requirement for Lyt-2 and Lyt-3 molecules on cytolytic T-lymphocytes: Analysis by antibody blocking and selective trypsinization, J. Exp. Med. 156:1711 (1982).PubMedCrossRefGoogle Scholar
  7. 7.
    E. Nakayama, E. Shiku, E. Stockert, H. F. Oettgen, and L. J. Old, Cytotoxic T-cells: Lyt phenotype and blocking of killing activity by Lyt-antisera, Proc. Natl. Acad. Sci. 76:1977 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    S. L. Swain, Significance of Lyt-phenotypes: Lyt-2 antibodies block activities of T-cells that recognize class 1 major histocompatibility complex antigens regardless of their function, Proc. Natl. Acad. Sci. 78:7101 (1981).PubMedCrossRefGoogle Scholar
  9. 9.
    D. P. Dialynas, M. R. Loken, A. L. Glasebrook, and F. W. Fitch, Lyt-2-/3- variants of a cloned cytolytic T-cell line lacks an antigen receptor functional in cytolysis, J. Exp. Med. 153:595 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    P. D. Gottlieb, Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen, J. Exp. Med. 140:1432 (1974).PubMedCrossRefGoogle Scholar
  11. 11.
    M. A. Palladino, J. G. Ranges, M. P. Scheid, and H. F. Oettgen, Suppression of T-cell cytotoxicity by nu/nu spleen cells: Reversal by monosaccharides and Interleukin-2, J. Immunol. 130:2200 (1983).PubMedGoogle Scholar
  12. 12.
    J. A. Ledbetter, W. E. Seaman, T. T. Tsu, and L. A. Herzenberg, Lyt-2 and Lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationships of subunits to T-cell cytolytic activity, J. Exp. Med. 153:1503 (1981).PubMedCrossRefGoogle Scholar
  13. 13.
    G. Jay, M. A. Palladino, G. Khoury, and L. J. Old, Mouse Lyt-2 antigen: Evidence for two heterodimers with a common subunit, Proc. Natl. Acad. Sci. 79:2654 (1982).PubMedCrossRefGoogle Scholar
  14. 14.
    J. Fan, A. Ahmed, and B. Bonavida, Studies on the induction and expression of T cell-mediated immunity. X. Inhibition by Lyt 2, 3 antisera of cytotoxic T lymphocyte-mediated antigen-specific and non-specific cytotoxicity: evidence for the blocking of the binding between T lymphocytes and target cells and not the post-binding cytolytic steps, J. Immunol. 125:2444 (1980).PubMedGoogle Scholar
  15. 15.
    E. Rothenberg and D. Triglia, Lyt-2 glycoprotein is synthesized as a single molecular species, J. Exp. Med. 157:365(1983).Google Scholar
  16. 16.
    S. M. Hendrick, E. A. Nielsen, J. Kavaler, D. I. Cohen, and M. M. Davis, Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins, Nature 308:155 (1984).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Michael A. PalladinoJr.
    • 1
    • 3
  • Gerald E. Ranges
    • 2
    • 3
  • Herbert F. Oettgen
    • 3
  • Lloyd J. Old
    • 3
  1. 1.Department of Pharmacological Sciences/ImmunologyGenentech, Inc.South San FranciscoUSA
  2. 2.Department of MedicineUniversity of Vermont College of MedicineBurlingtonUSA
  3. 3.Memorial Sloan Kettering Cancer CenterNew YorkUSA

Personalised recommendations