Abstract
Monoclonal antibodies (MAb) have provided powerful tools for dissecting the complex cellular interactions involved in the lysis of target cells by immune cytotoxic T lymphocytes (CTL). Seven distinct surface-membrane structures have been implicated to function in the cytolytic process mediated by human effector cells by virtue of the ability of their MAb to inhibit cytolysis in the absence of complement (Table 1). All of these inhibitory MAb recognize cell surface structures present on nonimmune mature lymphoid cells. The Ti, T3, T8 and T4 structures are unique to T cells and in the case for T8 and T4 define T-cell subpopulations that recognize antigen associated with Class I or Class II MHC glycoproteins, respectively (1–3). The clonotypic (Ti) surface structure and T3 appear to form the antigen binding recognition complex on the CTL surface (4) whereas the Lymphocyte Function Associated Antigen-2 (LFA-2) has been identified as the sheep red blood cell rosette receptor (5). In contrast, the LFA-1 molecule is present on both T and B lymphocytes and is structurally related to the OKM1/MAC-1 macrophage surface glycoprotein which is associated with complement receptor activity (CR3) (6,7). In contrast, the tissue distribution of LFA-3 is very broad including nonlymphoid cells (5).
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References
Swain, S.L. 1981. Significance of Lyt Phenotypes: Lyt2 Antibodies Block Activities of T Cells that Recognize Class 1 Major Histocompatibility Complex Antigens Regardless of Their Function. Proc. Natl. Acad. Sci. USA, 78;7101.
Krensky, A.M., C.S. Reiss, J.W. Mier, J.L Strominger and S.J. Burakoff. 1982. Long-term Human Cytolytic T-Cell Lines Allospecific for HLA-DR6 Antigen Are OKT4+. Proc. Natl. Acad. Sci. USA, 79:2365.
Ball, E.J. and P. Stastny. 1982. Cell-Mediated Cytotoxicity Against HLA-D-Region Products Expressed in Monocytes and B Lymphocytes. IV. Characterization of Effector Cells Using Monoclonal Antibodies Against Human T-Cell Subsets. Immunogenetics, 16:157.
Meuer, S.C., K.A. Fitzgerald, R.E. Hussey, et al. 1983. Clonotypic Structures Involved in Antigen-Specific Human T Cell Function: Relationship to the T3 Molecular Complex. J. Exp. Med., 157:705.
Krensky, A.M., F. Sanchez-Madrid, E. Robbins, J. Nagy, T.A. Springer and S.J. Burakoff. 1983. The Functional Significance, Distribution, and Structure of LFA-1, LFA-2, and LFA-3: Cell Surface Antigens Associated with CTL-Target Interactions. J. Immunol., 131:611.
Sanchez-Madrid, F., A.M. Krensky, C.F. Ware, E. Robbins, J.L. Strominger, S.J. Burakoff, and T.A. Springer. 1982. Three Distinct Antigens Associated with Human T Lymphocyte-mediated Cytolysis: LFA-1, LFA-2, LFA-3. Proc. Natl. Acad. Sci., USA, 79:7489.
Sanchez-Madrid, F., J.A. Nagy, E. Robbins, P. Simon and T.A. Springer. 1983. Characterization of a Human Leukocyte Differentiation Antigen Family with Distinct Subunits and a Common Subunit: The Lymphocyte Function-Associated Antigen (LFA-1), the C3bi Complement Receptor (OKMl/Mac-1), and the pl50,95 Molecule. J. Exp. Med., 158:1785.
Davignon, D., E. Martz, T. Reynolds, K. Kurzinger and T.A. Springer. 1981. Lymphocyte Function-Associated Antigen 1 (LFA-1): A Surface Antigen Distinct from Lyt-2,3 that Participates in T Lymphocyte-Mediated Killing. Proc. Natl. Acad. Sci. USA, 78:4535.
Krensky, A.M., E. Robbins, T.A. Springer and S.J. Burakoff. 1984. LFA-1, LFA-2, and LFA-3 Antigens Are Involved in CTL-Target Conjugation. J. Immunol., 132:2180.
Tsoukas, C.D., D.A. Carson, S. Fong and J.H. Vaughan. 1982. Molecular Interactions in Human T Cell-Mediated Cytotoxicity to EBV. II. Monoclonal Antibody OKT3 Inhibits a Post-Killer Target Recognition/Adhesion Step. J. Immunol., 129:1421.
Landegren, U., U. Ramstedt, I. Axberg, M. Ullberg, M. Jondal and H. Wigzell. 1982. Selective Inhibition of Human T Cell Cytotoxicity at Levels of Target Recognition or Initiation of Lysis by Monoclonal OKT3 and Leu-2a Antibodies. J. Exp. Med., 155:1579.
Ware, C.F., and G.A. Granger. 1981. Mechanisms of Lymphocyte Mediated Cytotoxicity. III. Characterization of the mechanism of inhibition of the human alloimmune cytotoxic reaction by the polyspecific anti-Iymphotoxin serum in vitro. J. Immunol., 126:1934.
Ware, C.F., P.H. Chauvenet, P.S. Duffey, and G.A. Granger. 1981. Inhibition of the Lytic Phase of Murine T-cell Mediated Alloimmune Cytotoxicity by a Rat Anti-activated T-cell Antiserum. Cell. Immunol., 59:289.
Springer, T.A., D. Davignon, M.-K. Ho, K. Kurzinger, E. Martz and F. Sanchez-Madrid. 1982. LFA-1 and Lyt-2,3, Molecules Associated with T Lymphocyte-Mediated Killing; and Mac-1, an LFA- 1 Homologue Associated with Complement Receptor Function. Immunological Reviews, 68:171.
Ware, C.F., F. Sanchez-Madrid, A.M. Krensky, S.J. Burakoff, J.JL. Strominger and T.A. Springer. 1983. Human Lymphocyte Function Associated Antigen-1 (LFA-1): Identification of Multiple Antigenic Epitopes and Their Relationship to CTL- Mediated Cytotoxicity. J. Immunol., 131:1182.
Malissen, B., N. Rebai, A. Liabeuf and C. Mawas. 1982. Human Cytotoxic T Cell Structures Associated with Expression of Cytolysis. I. Analysis at the Clonal Cell Level of the Cytolysis-Inhibiting Effect of 7 Monoclonal Antibodies. Eur. J. Immunol., 12:739.
Ware, C.F., M.S. Krangel, D. Pious, S.J. Burakoff and J.L. Strominger. 1983. Recognition of HLA-A2 Mutant and Variant Target Cells by an HLA-A2 Allospecific Human Cytotoxic T Lymphocyte Line. J. Immunol., 131:1312.
Yelton, D.E., C. Desaymard and M.D. Scharff. 1981. Use of Monclonal Anti-Mouse Immunoglobulin to Detect Mouse Antibodies. Hybridoma, 1: 5.
Ware, C.F., J.L. Reade and L.C. Der. 1984. A Rat Anti-Mouse Kappa Chain Specific Monoclonal Antibody, 187.1.1.0: Purification, Immunochemical Properties and Its Utility as a General Second-Antibody Reagent. J. Immunol. Method., (submitted).
Brodsky, F.M. and P. Parham. 1982. Monomorphic Anti-HLA-A, B, C Monoclonal Antibodies Detecting Molecular Subunits and Combinatorial Determinants. J. Immunol., 128:129.
Sande, M.A. and G.L. Mandell. 1980. In: The Pharmacological Basis of Therapeutics. Gilman, A.G., L.S. Goodman and A. Gilman (Eds.). MacMillan and Co., New York, pp. 1170.
MacDonald, H.R., A.L. Glasebrook, C. Bron, A. Kelso and J.-C. Cerottini. 1982. Clonal Heterogeneity in the Functional Requirement for Lyt-2/3 Molecules on Cytolytic T Lymphocytes (CTL): Possible Implications for the Affinity of CTL Antigen Receptors. Immunological Rev., 68:89.
Biddison, W.E., P.E. Rao, M.A. Talle, G. Goldstein and S. Shaw. 1984. Possible Involvement of the T4 Molecule in T Cell Recognition of Class II HLA Antigens: Evidence from Studies of CTL- Target Cell Binding. J. Exp. Med., 159:783.
Hemler, M.E., C.F. Ware and J.L. Strominger. 1983. Characteriza tion of a Novel Differentiation Antigen Complex Recognized by a Monoclonal Antibody (A-1A5): Unique Activation-Specific Molecular Forms on Stimulated T Cells. J. Immunol., 131:334.
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© 1985 Plenum Press, New York
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Ware, C.F., Reade, J.L. (1985). Functional Interactions of LFA-1, T8 and Alloantigen Surface Structures in T-Cell Mediated Cytotoxicity. In: Henkart, P., Martz, E. (eds) Mechanisms of Cell-Mediated Cytotoxicity II. Advances in Experimental Medicine and Biology, vol 184. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8326-0_22
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DOI: https://doi.org/10.1007/978-1-4684-8326-0_22
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