The Function of LFA-1 in Cell-Mediated Killing and Adhesion: Studies on Heritable LFA-1, Mac-1 Deficiency and on Lymphoid Cell Self-Aggregation
Lymphocyte function associated antigen-1 (LFA-1) is a cell surface glycoprotein identified in mouse and human by monoclonal antibodies which inhibit cytolytic T lymphocyte (CTL) mediated cytolysis (1–5). LFA-1 contains noncovalently associated a and 3 subunits of Mr = 180,000 and 95,000, respectively. Anti-LFA-1 monoclonal antibodies (MAb) inhibit cytolysis by CTL and NK cells as well as proliferative responses to mitogens, alloantigens, and soluble antigens. LFA-1 is broadly distributed on leukocytes, including lymphocytes, granulocytes, and monocytes, but is not expressed on a number of other somatic cells. Anti-LFA-1 monoclonal antibodies inhibit conjugate formation between CTL and targets (1, 6, 7); therefore, LFA-1 may be instrumental in cell-cell adhesion.
KeywordsBeta Chain Adhesion Step Mediate Cytolysis Deficient Lymphocyte Immunofluorescence Flow Cytometry
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