The Lipoxygenase Pathway in the Human NK Cell System
Induction of cell-mediated cytotoxicity requires trans-membrane signalling as in other lymphocyte activation systems. Earlier studies have pointed to the importance of arachidonic acid (AA) metabolites in regulating different cytotxicity systems (l–4a+4b). In the human NK cell system Seaman recently showed that antagonists of lipoxygenation such as nordihydroquaiaretic acid (NDGA) and 3-amino-l-m-(trifluoromethyl)-phenyl-2-pyrazoline (BW 755C) rapidly supress killing (5) and Suthanthiran et al that other anti-oxidants which scavenge hydroxyl radicals are suppressive (6). Hattori et al have demonstrated that purified lipomodulin, which selectivly inhibit phospholipase A2 activity, also inhibit NK cell killing (7). Using virally infected adenoma cells as target cells, Rola-Pleszynski demonstrated induction of NK cell activity with leukotriene B4 (LTB4) (8).
KeywordsNatural Killer Natural Killer Cell Natural Killer Cell Cytotoxicity Human Natural Killer Cell Lipoxygenase Product
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- 3.Berke G. Recent advances and questions in lymphocytotoxicity. In: Regulatory Mechanisms in Lymphocyte Activation. Ed E. Lucas. Academic Press, NY p 812, 1977.Google Scholar
- 4.Y Kobayashi, J-I Sawada and T Osawa. Activation of membrane phoshpolipase by guinea pgi lymphotoxin (GLT). J Immunol 122:1791, 1979.Google Scholar
- U Ramstedt, C N Serhan, U Lundberg, H Wigzell and B Samuelsson. Inhibition of human natural killer cell activity by 14,15- dihydroxy-4, 8, 10, 12-eicosatetraenoic acid (14, 15-D1HETE) PNS in press.Google Scholar
- 15.Shenker and I Grey. Cyclic nucleotide metabolism during lymphocyte transformation. 43: 11, 1979.Google Scholar
- 32.Serhan C N, Hamberg M, and Samuelsson B. Trihydroxytetraenes: A novel series of compounds formed from arachidonic acid in human leukocytes, 118:943, 1984.Google Scholar
- 33.Goldman D W and Goetzl E J. Eur J Immunol, 129:1600, 1983.Google Scholar
- 35.Coffey R G and Hadden J W. Arachidonate and metabolites in mitogen activation of lymphocyte guanylate cyclase. Advances in Immunopharmacol, Pergamon Press, 1981.Google Scholar