An Investigation of the Role of Soluble Cytotoxic Factors and Reactive Oxygen Intermediates in Lysis by NK Cells
The mechanism (s) by which cytotoxic T lymphocytes (CTL) and natural killer (NK) cells lyse sensitive target cells is one of the basic questions in immunology that has remained unanswered. In the last 2– years, two hypotheses concerning the mechanism of cytolysis by NK cells have attracted considerable attention. According to the first hypothesis, a stable soluble cytotoxin (NKCF) is released by NK cells during their interaction with target cells, and this factor acting alone is capable of target cell lysis (Wright and Bonavida, 1982; 1983). In the second hypothesis, a crucial role for reactive oxygen intermediates (ROI) was suggested by the observation of luminol chemiluminescence (CL) during interaction between NK-enriched populations and target cells (Roder et al., 1982), and the inhibition of lysis by superoxide dismutase (SOD; Roder et al., 1982), or hydroxyl radical (OH.) scavengers (Suthanthiran et al., 1984). Whether the ROI directly inflicted damage on the target cells, as is the case in some macro phage/neutrophil cytocidal systems (Nathan et al., 1979a; b), was unclear.
KeywordsNatural Killer Natural Killer Cell Natural Killer Activity Reactive Oxygen Intermediate Peritoneal Exudate Cell
Unable to display preview. Download preview PDF.
- Anbar, M. and Neta, P. 1967. A compilation of specific rate constants for the reactions of hydrated electrons, hydrogen atoms, and hydroxyl radicals with inorganic and organic compounds in aqueous solution. Int. J. Appl. Rad. Isotopes, 18: 493.Google Scholar
- De Luca, M. A. (ed.) 1978. Biolumineseence and chemiluminescence. Methods in Enzymology. Vol. LVIL Academic Press, New York.Google Scholar
- Dorfman, L. M. and Adams, G. E. 1973. Reactivity of the hydroxyl radical in aqueous solutions. NSRDS, National Bureau of Standards, 46, (Washington, D.C.).Google Scholar
- Kay, H. D., Smith, D. L., Sullivan, G. Mandell, G. L., and Donowitz, G. R. 1983. Evidence for a non-oxidative mechanism of human natural killer (NK) cell cytotoxicity by using mononuclear effector cells from healthy donors and from patients with chronic granulomatous disease. J. Imminol., 131:1784.Google Scholar
- Lozio, C. B. and Lozio, B. B. 1975. Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood, 45:321.Google Scholar